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We narrowed to 5,521 results for: crispr cas9 grna plasmid

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Showing: 61 - 90 of 5521 results

  1. p426_Cas9_gRNA-R-ARS607c

    Plasmid
    #87409
    Purpose
    p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87409-plasmid-map-sequence-id-174884

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  2. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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  3. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  5. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  6. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  10. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    LP1B and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110624-plasmid-map-sequence-id-211314

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  13. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

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  16. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110626-plasmid-map-sequence-id-208002

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  17. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid
    #136651
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136651-plasmid-map-sequence-id-273320

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  18. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  19. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  20. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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  25. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  26. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  27. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  29. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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Showing: 61 - 90 of 5521 results