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iCAP-ROOT

Plasmid

#192262

Purpose

AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

Depositor

Li Ye

Article

Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

Insert

Synthetic construct for ROOT VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-Pericam

Plasmid

#87381

Purpose

Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

Mito-Pericam [ratiometric]

Use

Retroviral

Expression

Mammalian

Promoter

Retroviral 5' LTR promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9

Plasmid

#135964

Purpose

Expresses SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV9.452sub.LUNG1

Plasmid

#184592

Purpose

non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection

Depositor

Viviana Gradinaru

Article

Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26

Insert

Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.AX

Plasmid

#195218

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

Depositor

Viviana Gradinaru

Article

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

Insert

Synthetic construct isolate AAV-PHP.AX VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sp-HPV-TM(ICAM)

Plasmid

#196510

Purpose

Express HPV antigen epitope with ICAM transmembrane domain

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

a HPV epitope with CD8a stalk linker and ICAM TM domain

Use

Lentiviral

Tags

3xflag tag

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-WT

Plasmid

#17608

Purpose

Mammalian expression of HA tagged ubiquitin

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.CAP-Mac

Plasmid

#200658

Purpose

Synthetic construct isolate AAV.CAP-Mac VP1 gene

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

Synthetic construct isolate AAV.CAP-Mac VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid insertion after Q588 of the AAV9 CAP…

Promoter

p41

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Article

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (Zeocin)

Species

Escherichia coli

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only