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Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
UseTagsHis-SUMO and Sortase tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorInsertHIS-HA-CMYC-p53 (TP53 Human)
UseTagsHIS-HA-CMYCExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
RBD#4L
Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP C RRMExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C93A
Plasmid#133910PurposeBacterial expression of His6-Ubc9-C93ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 93 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (1-366)
Plasmid#193039PurposeBacterial expression plasmid for full-length IST1 (1-366).DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (81-165)
Plasmid#193032PurposeBacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 81-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (1-75)
Plasmid#193031PurposeBacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 1-75PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP7 366-453
Plasmid#104598PurposeExpresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12DepositorInsertCHMP7 residues 366-453 (CHMP7 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTVKRSF225
Plasmid#135005PurposeMusF2 prenyltransferases from Nmu. PCC 7906DepositorInsertMusF2 prenyltransferaseM
UseTagsSUMO fusionExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF227
Plasmid#135006PurposeMusF1 prenyltransferase from Nostoc sp. UHCC 0398DepositorInsertMusF1 prenyltransferase
UseTagsSUMO fusionExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-rM1PYK
Plasmid#220232PurposeInducible expression of rabbit muscle pyruvate kinase isoform 1DepositorInsertRabbit muscle pyruvate kinase (PKM )
UseTags6xHis-MBP-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-Smt-IIShhN
Plasmid#121135PurposeExpresses N-terminal SUMO tagged II-ShhN in bacteriaDepositorInsertShh (Shh Mouse)
UseTagsHIS, HRV3C site, MBP, and Smt tagExpressionBacterialMutationAmino acid sequence from 26-195, with two extra i…PromotertacAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA528 huPQBP1
Plasmid#217449PurposeExpresses full-length human PQBP1 from a His-SUMO bacterial expression vectorDepositorInsertPQBP1 (PQBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (322-366)
Plasmid#193038PurposeBacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 322-366PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L328D, L355A (1-366)
Plasmid#193040PurposeBacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationL328D, L355APromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 L61D (1-165)
Plasmid#193033PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutationDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationL61D, Residues 1-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 V18D,F98D (1-165)
Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 316-366, C-Cys
Plasmid#191234PurposeBacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labelingDepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 316-366, contains nonnative C-terminus f…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C 156-233
Plasmid#108257PurposeExpresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9DepositorInsertCHMP4C residues 156-233 (CHMP4C Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP5 159-222
Plasmid#108259PurposeExpresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10DepositorInsertCHMP5 residues 148-219 (CHMP5 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1A 140-196
Plasmid#104593PurposeExpresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1DepositorInsertCHMP1A residues 140-196 (CHMP1A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2A 152-222
Plasmid#104594PurposeExpresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3DepositorInsertCHMP2A residues 152-222 (CHMP2A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2B 141-213
Plasmid#104595PurposeExpresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4DepositorInsertCHMP2B residues 141-213 (CHMP2B Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP6 145-201
Plasmid#104597PurposeExpresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11DepositorInsertCHMP6 residues 145-201 (CHMP6 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only