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Showing: 1161 - 1200 of 96697 results

  2. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  23. pN1mcherry-NKD2G2A

    Plasmid
    #174334
    Purpose
    encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    mutation of a.a 2 Gly to Ala
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174334-plasmid-map-sequence-id-349873

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  24. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174333-plasmid-map-sequence-id-349872

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  25. FG11F-C-FLAG-hNKD2

    Plasmid
    #174330
    Purpose
    encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174330-plasmid-map-sequence-id-349820

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  26. MO70-C-FLAG-hNKD2

    Plasmid
    #174327
    Purpose
    encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Retroviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174327-plasmid-map-sequence-id-349813

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  30. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

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  40. pLentiRNAGuide_003

    Plasmid
    #192505
    Purpose
    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192505-plasmid-map-sequence-id-375155

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Showing: 1161 - 1200 of 96697 results