We narrowed to 19,273 results for: Met;
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Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS
Plasmid#140013PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
ExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395AAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTST5
Plasmid#86602PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST5
UseInternal standard reverse transcriptionPromoterT7Available SinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTST6
Plasmid#86603PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST6
ExpressionBacterialPromoterT7Available SinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-MCRaeo-ND
Plasmid#192771PurposeExpresses ND-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NC
Plasmid#192770PurposeExpresses NC-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBGA
Plasmid#192769PurposeExpresses NG-tagged MCRaeoBGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBCGA
Plasmid#192768PurposeExpresses NG-tagged MCRaeoBCGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBDGA
Plasmid#192767PurposeExpresses NG-tagged MCRaeoBDGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-CA
Plasmid#192765PurposeExpresses CA-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NB
Plasmid#192764PurposeExpresses NB-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG
Plasmid#192763PurposeExpresses NG-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
TagsV5-His6ExpressionMammalianPromoterCMV and U6/H1Available SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP 150TAG
Plasmid#197570PurposeExpression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
Pyl-tRNA (4 copies)
TagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaDXMT
Plasmid#139165PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.DepositorInsert3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
UseTransposonExpressionMammalianPromoterEF1αAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaMXMT
Plasmid#139172PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.DepositorInsertmonomethylxanthine methyltransferase 1 [Coffea arabica]
UseTransposonExpressionMammalianPromoterEF1αAvailabilityAcademic Institutions and Nonprofits only -
pCfB13089
Plasmid#212676PurposeTEF promoterDepositorInsertTEF
ExpressionYeastAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only