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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB3

Plasmid

#23874

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB3 (ERBB3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pminiTol2

Plasmid

#31829

Purpose

Vector with minimal Tol2 transposition sites, with capacity for delivering larger gene cargo than standard Tol2 vectors

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Expression

Bacterial

Promoter

T7

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2A

Plasmid

#24180

Purpose

Mammalian expression of human JMJD2A with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2A (KDM4A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pTight-9-124-BclxL

Plasmid

#60857

Purpose

2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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CA-NFAT2

Plasmid

#11102

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Mutation

Constitutively active: multiple serine to alanine…

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

Plasmid

#64613

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

V5

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6-DEST PINK1-V5 WT

Plasmid

#13320

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-HA-BAP1

Plasmid

#22539

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-UtrCH

Plasmid

#26740

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

mCherry

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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