We narrowed to 17,875 results for: puromycin

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pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Publication

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled library

#83978

Purpose

Version 2 of the human CRISPRa genome wide library

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles
Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled library

#67989

Purpose

Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles
Flag-HA-USP13

Plasmid

#22568

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP13 (USP13 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Bison sgRNA library

Pooled library

#169942

Purpose

Knockout library targeting ubiquitin ligases, deubiquitinases, and control genes.

Depositor

Benjamin Ebert

Publication

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Use

Lentiviral

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Publication

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-IP GFP-NBR1

Plasmid

#38283

Depositor

Noboru Mizushima

Publication

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Next to BRCA1 gene 1 protein (NBR1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Publication

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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ClonTracer Barcoding Library (2.5 ug)

Pooled library

#67267

Purpose

High complexity 30bp-long semi random DNA barcode library in lentiviral backbone

Depositor

Frank Stegmeier

Publication

Bhang et al Nat Med. 2015 Apr 13. doi: 10.1038/nm.38

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
pSLQ1661-sgMUC4-E3(F+E)

Plasmid

#51025

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

Depositor

Bo Huang, Stanley Qi

Publication

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

optimized sgRNA (MUC4 Mouse, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Publication

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

T790M

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Publication

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Publication

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Publication

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pMSCV-U6gRNA(lib)-PGKpuroT2ABFP library

Pooled library

#104861

Purpose

Retroviral library containing gRNAs from the Yusa lab Mouse improved genome-wide library v2.

Depositor

Sarah Teichmann

Publication

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles