We narrowed to 17,461 results for: She
-
Plasmid#222201PurposeBacterial expression of mouse PTER-T258G proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationT258GPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-R304K
Plasmid#222202PurposeBacterial expression of mouse PTER-R304K proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationR304KPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-Y65F
Plasmid#222203PurposeBacterial expression of mouse PTER-Y65F proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationY65FPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-Y263F
Plasmid#222204PurposeBacterial expression of mouse PTER-Y263F proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationY263FPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1_RBFOX2_RRM
Plasmid#224293PurposeGST-RBFOX2(RRM domain) purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 RNA-recognition motif (RBFOX2 Human)
UseTagsGSTExpressionBacterialMutationaa 74-167Promotertac promoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LMPd Ametrine Got1 shRNA#1
Plasmid#220592PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot1 shRNA (Got1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK189
Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7183
Plasmid#219760PurposeMoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)DepositorInsertPzPKS2
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7180
Plasmid#219762PurposeMoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)DepositorInsertHmS
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
UseTagsExpressionYeastMutationDHFR (P66L), Rho1 (C17R)PromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro 2xStrep-HPC4-Gb5
Plasmid#218177PurposeUsed to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cellsDepositorInsertG beta subunit 5 (GNB5 Human)
UseLentiviralTags2x Strep tag and HPC4ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
Plasmid#210502Purposeexpresses LAUNCHERtvmv component in mammalian cellsDepositorInsertPDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Creb5 shRNA
Plasmid#195020PurposeBovine Creb5 shRNA targeting the Creb5 3′UTRDepositorInsertCreb5 shRNA (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) T59/T61 A
Plasmid#195032PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationT59/T61 APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) C18/C23 S
Plasmid#195033PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationC18/C23 SPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR + 5M→A_CR
Plasmid#194268PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CRDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M M322A, M323A, M…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_gRNA_3418711_KCNB2
Plasmid#197558PurposegRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)DepositorInsertupstream KCNB2 promoter gRNA (Kcnb2 Rat)
UseGrna with puromycin selectionTagspuromycin resistance cassette under CMV promotionExpressionMammalianMutationPromoterU6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sox2AV
Plasmid#193354PurposeRetroviral vector expressing mouse Sox2A61V point mutantDepositorInsertSox2AV (Sox2 Mouse)
UseRetroviralTagsExpressionMammalianMutationSox2A61V is a highly cooperative point mutant of …Promoter5'LTRAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2AV
Plasmid#193352PurposeRetroviral vector expressing human SOX2A61V point mutantDepositorInsertSOX2AV (SOX2 Human)
UseRetroviralTagsExpressionMammalianMutationSOX2A61V is a highly cooperative point mutant of …Promoter5'LTRAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S1dC2
Plasmid#183769PurposeExpresses SHIP1-delta-C2-Enzyme domain flanked by RBPDepositorInsertSHIP1deltaC2 (INPP5D Human)
UseTags6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…ExpressionBacterialMutationSHIP1deltaC2 includes aa N393 to aa Q730 onlyPromoterT7lacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP1
Plasmid#183770PurposeExpresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP1 (INPP5D Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa1 to aa883 onlyPromoterT7 lacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S1-Enz
Plasmid#183767PurposeExpresses SHIP1-Enzyme domain flanked by RBPDepositorInsertSHIP1 (INPP5D Human)
UseTags6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…ExpressionBacterialMutationincludes aa N393 to aa L864 onlyPromoterT7lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP2
Plasmid#183771PurposeExpresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP2 (INPPL1 Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa18 to aa985 onlyPromoterT7 lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
UseTagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pSico_U6-PGC1a1 sgRNA
Plasmid#165425PurposeExpression of gRNA against human PGC-1a variant 1DepositorInsertgRNA against PGC-1a variant 1 (PPARGC1A Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.DelHNS IRES Puro
Plasmid#110389Purposeγ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-DelFSH(307-309)-pKK223
Plasmid#131377PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.DepositorInsertEcNGsC MutY chimera DelFSH(307-309)
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308P-H309D-pKK223
Plasmid#131378PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.DepositorInsertEcNGsC MutY chimera S308P H309D
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-S308A-pKK223
Plasmid#131379PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A S308A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A
Plasmid#112987PurposeIn P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genomeDepositorInsertmei-S332 (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationS123-125-3APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D
Plasmid#112985PurposeIn P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genomeDepositorInsertmei-S332 (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationS123-125-3DPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15D
Plasmid#113010PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutationsDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to DPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO6206
Plasmid#97065PurposeExpressing Sulfolobus solfataricus orf SSO6206 proteinDepositorInsertS. solfataricus orf SSO6206
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOG40-HRS (257-276) Hs
Plasmid#83236PurposepGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coliDepositorInsertHRS (257-276) Hs (HGS Human)
UseTagsExpressionBacterialMutation258-277PromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK215
Plasmid#72427PurposeAcetobacter aceti 1023 gDNADepositorInsertssuccinyl-diaminopimelate desuccinylase
2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
acetylglutamate kinase
ribosome biogenesis GTP-binding protein YsxC
insertase
ribonuclease P
50S ribosomal protein L34
hypothetical protein
glyoxylase I
hypothetical protein
UDP-N-acetylglucosamine acyltransferase
3-hydroxyacyl-ACP dehydratase
UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
UsePhage lambda cloning vector with genomic dna inse…TagsExpressionMutationmissing C-terminal domain residues 277-391(ter) a…Promotern/aAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only