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We narrowed to 6,406 results for: siae

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Showing: 5981 - 6000 of 6406 results

  2. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

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  4. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Joan Conaway
    Article
    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    175949-plasmid-map-sequence-id-345352

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  5. pIRHDGAL43C

    Plasmid
    #218279
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218279-plasmid-map-sequence-id-433681

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  6. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218276-plasmid-map-sequence-id-433677

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  11. Coexpressed VLP NES

    Plasmid
    #182433
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182433-plasmid-map-sequence-id-360116

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  12. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182435-plasmid-map-sequence-id-360119

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  13. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182477-plasmid-map-sequence-id-360429

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  14. Coexpressed Free PcPTS

    Plasmid
    #182483
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    PTS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182483-plasmid-map-sequence-id-360444

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  16. Linked Free (PT)4P

    Plasmid
    #182490
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182490-plasmid-map-sequence-id-360448

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  17. deltaVP1 + VP2C-MIOX

    Plasmid
    #166680
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166680-plasmid-map-sequence-id-329927

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  18. wtVP1 + VP2C-GFP

    Plasmid
    #166674
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166674-plasmid-map-sequence-id-329826

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  19. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid
    #166855
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    K683E, H685E, K686E
    Promoter
    pCu
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166855-plasmid-map-sequence-id-329426

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  20. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid
    #166854
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    F641E, I645E
    Promoter
    pCu
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166854-plasmid-map-sequence-id-329424

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Showing: 5981 - 6000 of 6406 results