We narrowed to 6,481 results for: siae
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Plasmid#202090PurposeNeuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutationsDepositorInsertdCas9-Set2(R195G, C201A)
Tags3x FLAG and mCherryExpressionMammalianMutationR195G and C201A abolish catalytic activityPromoterhuman SynapsinAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
Plasmid#212805PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
ExpressionMammalianAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1
Plasmid#179923PurposeExpression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertFvPPT1
ExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_591
Plasmid#160653PurposeExpresses OLE1 under the control of the yeast Sup35 promoterDepositorAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
TagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_2_dCas9_VP64_sgRNA
Plasmid#163707PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domainDepositorInsertsdCas9
VP64+SV40 NLS
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyExpressionYeastPromoterGAL10Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3G919C
Plasmid#120255PurposeExpression of ATP3-7 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationG919 changed to C; Alanine 307 changed to ProlinePromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3mut
Plasmid#120256PurposeExpression of ATP3-67 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationIsoleucine 304 changed to Asparagine; Alanine 307…PromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C
Plasmid#81239PurposeU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPK-352
Plasmid#157922PurposepcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNRDepositorInsertstPcyA
IRES
HO1
FD
FNR
UseSynthetic Biology; OptogeneticsTagsMitochondrial Targeting Sequence, Mitochondrial T…ExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only