We narrowed to 15,153 results for: RING;

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ptRNA100

Plasmid

#154133

Purpose

Expresses a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

tRNAs operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9

Plasmid

#243744

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_HUHe-Pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDH42(CUG/A)

Plasmid

#154132

Purpose

Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

engineered bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-TEX264-GFP

Plasmid

#201925

Purpose

For lentiviral transduction of TEX264-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPN-mKate (PT7LacO-PN-mKate)

Plasmid

#191126

Purpose

Expression of protein N tagged with mKate from a T7-lac inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

Protein N

Use

Synthetic Biology

Tags

mKate

Promoter

T7-lac inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-I-SceI-Trex2

Plasmid

#44024

Depositor

Jeremy Stark

Article

Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

Insert

I-SceI fused to mTREX2

Tags

NLS-HA

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pT2A-EKAREV-NLS

Plasmid

#173856

Purpose

Encoding EKAREV-NLS.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

EKAREV-NLS

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-EKARrEV-NLS

Plasmid

#173854

Purpose

A lentiviral vector for EKARrEV-NLS

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

EKARrEV-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Expression

Bacterial

Promoter

N/A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART-SOS-GFPuv

Plasmid

#102283

Purpose

Fluorescent sensor of E. coli SOS response

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

cda-GFPuv

Expression

Bacterial

Promoter

cda SOS promoter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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XE68 APC-GFP in CS2+

Plasmid

#16687

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

APC-GFP (APC Human)

Use

Xenopus expression

Tags

GFP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-NICD1

Plasmid

#46048

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Gateway Cloning

Tags

Myc tag

Mutation

Cytoplasmic domain initiating at amino acid 1753

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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