We narrowed to 10,418 results for: 158

1. hU6-pegRNA-EFS-mScarlet Template

   Plasmid

   #196037

   Purpose

   The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and EFS

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_SPOP_p.F102C

   Plasmid

   #81642

   Purpose

   Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SPOP (SPOP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   F102C

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-RKIP_RFP

   Plasmid

   #199215

   Purpose

   RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Inserts

   RKIP (PEBP1 Human)
   RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and PGK

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RFP-KIF1B beta-FL

   Plasmid

   #79111

   Purpose

   Mammalian expression of RFP-KIF1B beta-FL

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   RFP-KIF1B beta full length (KIF1B Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_SPOP_p.E50K

   Plasmid

   #81631

   Purpose

   Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SPOP (SPOP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   E50K

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDY0207 ACTB atgRNA with v1 scaffold

   Plasmid

   #179107

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_TBL1XR1_WT

   Plasmid

   #82059

   Purpose

   Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TBL1XR1 (TBL1XR1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   L512P

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_SPOP_p.K101I

   Plasmid

   #81641

   Purpose

   Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SPOP (SPOP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   K101I

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_SPOP_p.W131C

    Plasmid

    #81643

    Purpose

    Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    W131C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_SPOP_p.E47A

    Plasmid

    #81645

    Purpose

    Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E47A

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_SPOP_p.K134N

    Plasmid

    #81647

    Purpose

    Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    K134N

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plenti-Lb-Cas12a-2xNLS

    Plasmid

    #155046

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_RBM10_WT

    Plasmid

    #81958

    Purpose

    Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJV137

    Plasmid

    #124256

    Purpose

    Lentiviral vector (pRRL) containing Her2-ITD specific T cell receptor

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    T cell receptor (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CYLBL_Neo_CAGdCas9-KRAB

    Plasmid

    #220439

    Purpose

    CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

    Depositor

    Yin Shen

    Article

    Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

    Insert

    dCas9
    

    Use

    CRISPR and TALEN

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223_PIK3R3_WT

    Plasmid

    #81903

    Purpose

    Gateway Donor vector containing PIK3R3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3R3 (PIK3R3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D338A

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_016-sgATF4-1

    Plasmid

    #202455

    Purpose

    Expression of a guide RNA targeting ATF4

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    ATF4 gRNA (ATF4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid

    #208957

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid

    #217804

    Purpose

    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLX_314-HRI-V5

    Plasmid

    #202434

    Purpose

    Expression of HRI

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDY0310 TP901-1 Integrase

    Plasmid

    #179110

    Purpose

    TP901-1 Integrase expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    TP901-1 Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTagGFP2-N SIN1 deltaCRIM

    Plasmid

    #124921

    Purpose

    For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTagGFP2-N SIN1 deltaPH

    Plasmid

    #124922

    Purpose

    For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid

    #208947

    Purpose

    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_XIAP_WT

    Plasmid

    #82170

    Purpose

    Gateway Donor vector containing XIAP , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    XIAP (XIAP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_MTM1_WT

    Plasmid

    #81908

    Purpose

    Gateway Donor vector containing MTM1 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MTM1 (MTM1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E410K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge