We narrowed to 66,338 results for: nin

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pCAG-Archon2-KGC-GFP-ER2-WPRE

Plasmid

#108424

Purpose

Archon2 fluorescent voltage reporter in mammalian expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hFOXH1

Plasmid

#59183

Purpose

Forced expression of human FOXH1

Depositor

Shinya Yamanaka

Publication

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

FOXH1 (FOXH1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Publication

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway Cloning

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Publication

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Mutation

truncation: aa 11-276

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Publication

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiGuide-Puro-P2A-EGFP_mRFPstuf

Plasmid

#137730

Purpose

Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

Depositor

Fredrik Wermeling

Publication

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

Expression

Bacterial and Mammalian

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma13-GFP2

Plasmid

#140992

Purpose

Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma13-GFP2 (GNG13 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

Plasmid

#100157

Purpose

Expresses MUS81

Depositor

Peter Howley

Publication

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

MUS81 (MUS81 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3

Plasmid

#108103

Purpose

Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Publication

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUb6-Zpo1-V5/His

Plasmid

#29323

Depositor

Zena Werb

Publication

Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

Insert

Zeppo1

Tags

His and V5

Expression

Mammalian

Available since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
CBX4

Plasmid

#25237

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX4_02 (CBX4 Human)

Tags

6x His and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles
-227 hTF

Plasmid

#15442

Depositor

Nigel Mackman

Publication

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pNJP

Plasmid

#115494

Purpose

Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Human, Mouse, Synthetic)

Tags

iRFP, mCherry, and mKO

Expression

Mammalian

Mutation

mCherry S227F, mCherry G229R, mKO V211G

Promoter

CAG

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-TripleColour-StAR

Plasmid

#222695

Purpose

Competition assay

Depositor

Ulrich Elling

Publication

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP, miRFP703, dTomato

Expression

Mammalian

Mutation

PGK, TagBFP, dTomato without BsmBI cutsite

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Expression

Mammalian

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

Plasmid

#187588

Purpose

Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Publication

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NLS

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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TPC1-mCherry

Plasmid

#135182

Purpose

Expresses TPC1 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Publication

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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