167,464 results

1. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV1)

   Viral Prep

   #26971-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   EYFP

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pXJ-Myc-GATA4

   Plasmid

   #232647

   Purpose

   transient expresses GATA4 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EF1a-fDIO-Cre (AAV9)

   Viral Prep

   #121675-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Promoter

   EF1a

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

4. AAVS1-Puro Xlone-eGFP

   Plasmid

   #136936

   Purpose

   donor plasmid for inducible transgene expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   all-in-one tet-on system
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   deletion of AA 1 to 45

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-EF1A-CD19.28H.28TM.BB.3z

   Plasmid

   #200679

   Purpose

   Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. rAAV9-CAP-in-cis-Lox-AAV

   Plasmid

   #205990

   Purpose

   rAAV9 cap acceptor vector for CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   rAAV9 cap acceptor vector for CREATE libraries
   

   Use

   AAV

   Mutation

   Deletion between XbaI and AgeI sites

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CaM/pIRES2-eGFP

   Plasmid

   #111499

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)

   Viral Prep

   #26976-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

10. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid

    #223952

    Purpose

    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EC1000

    Bacterial Strain

    #71852

    Purpose

    RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Bacterial Resistance

    Kanamycin

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CCR5-Tango

    Plasmid

    #66242

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGFPGUSPlus

    Plasmid

    #64401

    Purpose

    binary plant vector expressing GFP and GUS, both driven by a 35S promoter

    Depositor

    Claudia Vickers

    Article

    Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

    Inserts

    GFP
    GUSPlus
    

    Use

    Binary vector for plant transformation

    Tags

    His

    Expression

    Plant

    Mutation

    S65T

    Promoter

    35S

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-PDGFRb-IRES2-EGFP

    Plasmid

    #204354

    Purpose

    Expresses wild-type PDGFRb gene.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-SKII-3XHA-HALO-NAT

    Plasmid

    #188931

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge