We narrowed to 7,122 results for: poly
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Plasmid#249289PurposeSP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassetteDepositorInsertCHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes
ExpressionBacterialMutationstructural polyprotein open reading frame deleted…PromoterSP6Available SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
3922-48
Plasmid#176497PurposepTRE-EGFP-T2A-TFEB(wt)-polyADepositorInsertEGFP-T2A-TFEB(wild-type; full-length; human)
ExpressionMammalianPromoterTREAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1 E63A
Plasmid#112861Purposeplasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cellsDepositorArticleInsertAPOBEC1 (Apobec1 Rat)
ExpressionMammalianMutationchanged Glutamic acid 63 to AlaninePromoterCMV promoterAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
ExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
ExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Plasmid#176630PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell linesDepositorInsertEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX R139A
Plasmid#192058PurposeExpression vector for SOX (ORF37) R139ADepositorInsertSOX (ORF37)
ExpressionInsectMutationR139APromoterPolyhedrinAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
TagsStrep II tag on Cas1Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCherry
Plasmid#99265PurposeContains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmCherry
TagsH2BExpressionMammalianPromoterSP6Available SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#166229PurposeLentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.DepositorInsertmCherry-CAAX
UseLentiviralTagsCAAXExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3E5E_eGFP
Plasmid#69359PurposeEncodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsert3E5E eGFP minigenome
UseT7PromoterT7Available SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH6
Plasmid#129424PurposeExpression human MSH6 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
TagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectPromoterPolyhedrin (LRP6)/p10 (MESD)Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas3
Plasmid#178881PurposeExpression of human codon-optimized NlaCas3 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas3c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only