We narrowed to 81,643 results for: TRI

1. pLKO-shC

   Plasmid

   #21963

   Depositor

   Lynda Chin

   Article

   Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

   Insert

   si NEDD9 (NEDD9 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pENTR221 UBR5 ∆HECT

   Plasmid

   #81065

   Purpose

   Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
   

   Use

   Cloning

   Mutation

   C2768A (bp t8302g and g8303c)

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Tag4A-MMP8 Wt

   Plasmid

   #29545

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Venus-cpVenus-FLARE-Cameleon

   Plasmid

   #123350

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-Cameleon
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Venus-cpVenus-FLARE-ICUE

   Plasmid

   #123355

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-ICUE
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-AMVpl-δWT-nAchR

   Plasmid

   #97188

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Delta nicotinic acetylcholine receptor (Chrnd Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-AMVpl-γWT-nAchR

   Plasmid

   #97187

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Gamma nicotinic acetylcholine receptor (Chrng Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-p62-TIR-AAA

   Plasmid

   #187901

   Purpose

   Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Triple alanine mutant: P228A, E230A, P232A

   Promoter

   CMV

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123329

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN089

    Plasmid

    #91614

    Purpose

    Express sgRNA targeting human FURIN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    FURIN (FURIN Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

    Plasmid

    #149714

    Purpose

    Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC-BAR-YR

    Plasmid

    #61766

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

    Plasmid

    #161577

    Purpose

    Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid

    #191753

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1523A>T (p.K508M) + silent mutation c.1515A&…

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINB-hRNF216(511-784)

    Plasmid

    #171923

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRES2-EGFP-p53 tethered

    Plasmid

    #49245

    Purpose

    expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53-p53 (TP53 Human)
    

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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