We narrowed to 8,708 results for: sgRNA

1. px459-TDP-43 sgRNA1

   Plasmid

   #133762

   Purpose

   Expresses Cas9 and human TDP-43 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH-sgRNA1-2XPP7

   Plasmid

   #75390

   Purpose

   sgRNA1-2XPP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYLsgRNA-AtU3b/LacZ

   Plasmid

   #66199

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   AtU3b

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459-TDP 43 sgRNA2

   Plasmid

   #133763

   Purpose

   Expresses Cas9 and human TDP-43 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1651-sgRNA(F+E)-sgGal4

   Plasmid

   #100549

   Purpose

   sgGal4-expressing vector modified from Addgene plasmid #51024

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   sgGal4
   

   Use

   CRISPR

   Tags

   sgRNA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCAR_sgRNA_blast-tagBFP-lox5171

   Plasmid

   #162072

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCAR_sgRNA_hygro-tagBFP-lox5171

   Plasmid

   #162070

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCherry U6-sgRNA (BsmBI)

    Plasmid

    #214128

    Purpose

    Mammalian continuous expression

    Depositor

    Sho Yoshimatsu

    Insert

    sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCAR_sgRNA_hygro-tagBFP-lox2272

    Plasmid

    #162078

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6 SgRNA RBM20

    Plasmid

    #162735

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for RBM20
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-BFP-U6-sgRNA

    Plasmid

    #196725

    Purpose

    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYLsgRNA-OsU6a/LacZ

    Plasmid

    #66195

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU6a

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet1-sgRNA(siteA)

    Plasmid

    #154831

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteA)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCAR_sgRNA_blast-tagBFP-lox2272

    Plasmid

    #162071

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT9679-sgRNA-BFP

    Plasmid

    #162988

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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