We narrowed to 9,209 results for: sgRNA
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Plasmid#113738PurposeGateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR and Gateway CloningPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-PpU6P-sgRNA-L3L2
Plasmid#113740PurposeGateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR and Gateway CloningPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-PpU6P-sgRNA-L1L4
Plasmid#113736PurposeGateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR and Gateway CloningPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorPublicationInsertLoxP sgRNA
ExpressionMammalianAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRExpressionBacterialPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXPR_071
Plasmid#164558PurposeHigher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCKO_PSMD1_sgRNA_1
Plasmid#74180Purposelentiviral vector expressing sgRNA targeting PSMD1DepositorInsertPSMD1 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWT055h
Plasmid#96867PurposesgRNA-HEK4DepositorInsertsgRNA-HEK4
ExpressionMammalianAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
px459-Rheb sgRNA
Plasmid#133768PurposeExpresses Cas9 and human Rheb sgRNADepositorInsertRheb sgRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-GFP
Plasmid#200068PurposeFor the knock down of GFP with an astrocyte specific mCherry expressionDepositorInsertU6-sgRNA-GFP
UseAAV, CRISPR, and Mouse TargetingTagsGfaABC1D-mCherryPromoterU6, GfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-POLQ-Halo
Plasmid#211636PurposesgRNA against POLQDepositorInsertsgRNA POLQ
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_AAN_4
Plasmid#231415PurposeThis AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.DepositorInsertsdCas9
GFP
sgRNA-X
sgRNA-Y
UseSynthetic BiologyAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_sgRNA
Plasmid#68422PurposeTransient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertGluc sgRNA
UseCRISPRExpressionMammalianPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRExpressionInsectPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRExpressionInsectPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-NEAT1pr_v2
Plasmid#97082PurposeEncodes an sgRNA that creates a DSB at the promoter of human NEAT1 geneDepositorInsertsgRNA NEAT1pr_v2
UseCRISPRPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)
Plasmid#81246PurposeFor assaying self-targeting activity via Cas9DepositorPublicationInsertsgRNA
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only