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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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pLKO.1/CFP sh-hUBE2N

Plasmid

#246752

Purpose

Mammalian and lentiviral expression of shRNA targeting human UBE2N (Ubc13)

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

sh-hUBE2N (UBE2N Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL YD/FL

Plasmid

#214805

Purpose

Mammalian expression of human CALR wtΔKDEL YD/FL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR wtΔKDEL YD/FL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR del52-linker-Venus

Plasmid

#214788

Purpose

Mammalian expression of human CALR del52-linker-Venus

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR ins5-linker-Venus

Plasmid

#214789

Purpose

Mammalian expression of human CALR ins5 -linker-Venus

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL-FLAG-KDEL

Plasmid

#214792

Purpose

Mammalian expression of human CALR wtΔKDEL-FLAG-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALRwtΔKDEL-FLAG-KDEL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-G86R

Plasmid

#235663

Purpose

Express human SOD1-G86R in mammalian cells

Depositor

Chen Seng Ng

Article

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-G86R (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Glycine 86 to arginine

Promoter

CMV promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-D91A

Plasmid

#235664

Purpose

Express human SOD1-D90A in mammalian cells

Depositor

Chen Seng Ng

Article

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-D91A (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Aspartic acid 91 to alanine

Promoter

CMV promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-F97W

Plasmid

#203572

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Phenylalanine 97 to Tryptophan for partia…

Promoter

CMV

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-EZR

Plasmid

#227293

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of EZR (EZR Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor-R rich

Plasmid

#225478

Purpose

Express mEGFP-GAPDH-Tudor-R fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G142D (GAT)+deltaV143-Y145

Plasmid

#212459

Purpose

Encodes SARS-CoV-2 spike G142D, V143-Y145 deletion for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G142D, V143-Y145 deletion (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G142D, V143-Y145 deletion

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.12.1

Plasmid

#212503

Purpose

Encodes SARS-CoV-2 variant BA.2.12.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2.12.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.75.2

Plasmid

#212508

Purpose

Encodes SARS-CoV-2 variant BA.2.75.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2.75.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC) S373P (CCA) S375F (TTC) T376A (GCT)

Plasmid

#212493

Purpose

Encodes SARS-CoV-2 spike S371F, S373P, S375F, T376A for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S371F, S373P, S375F, T376A (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S371F, S373P, S375F, T376A

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

Plasmid

#212498

Purpose

Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q493R, Q498R, N501Y, Y505H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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