We narrowed to 12,024 results for: pcdna

1. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT to BioID-cDIC2-3xFlag

   Plasmid

   #232416

   Purpose

   Expresses human dynein subunit DIC2 in mammalian cells.

   Depositor

   Samara Reck-Peterson

   Article

   Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

   Insert

   Dynein Intermediate Chain 2 (DYNC1I2 Human)
   

   Use

   Tags

   BioID and Flag-3x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 T21R-Nb139-T2A-mCherry

   Plasmid

   #225589

   Purpose

   Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-Nb139-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3puro-mGFP-FXR1a-CC1mut (N202P)

   Plasmid

   #225455

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (N202P)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N202P

   Promoter

   CMV

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)

   Plasmid

   #231379

   Purpose

   Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

   Depositor

   David Bartel

   Article

   Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

   Insert

   Human Argonaute2 (AGO2 Human, Synthetic)
   

   Use

   Tags

   3xFLAG-SUMO_Eu1

   Expression

   Mammalian

   Mutation

   H56A, R68A, R97A, K98A

   Promoter

   CMV

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

   Plasmid

   #228532

   Purpose

   A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Skp1(dC111):gs10:FRB:HA (SKP1 Human, Synthetic)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-HA-GTSE1-S91, 262 ,724A

   Plasmid

   #224737

   Purpose

   For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Serine 91, 262 ,724 to Alanine

   Promoter

   CMV

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_eGFP-pScaC-MTS

   Plasmid

   #228827

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   MTS and eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3puro-EGFP-FXR1-C (379-621)

    Plasmid

    #225451

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (FXR1 fragment (379-621))

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C terminal fragment (379-621)

    Promoter

    CMV

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

    Plasmid

    #227614

    Purpose

    Overexpression of SLC25A46 in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

    Plasmid

    #227619

    Purpose

    Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    deleted aa 2-83

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

    Plasmid

    #209411

    Purpose

    Expression of wild-type adult isoform of human SCN8A.

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

    Insert

    SCN8A Variant 3, stabilized (SCN8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Modifed human HSPA5 intron 4 inserted after bp237…

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-FLAG-Regnase-3-HA_full-length

    Plasmid

    #222656

    Purpose

    Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-FLAG-Regnase-3_1-547 aa

    Plasmid

    #222657

    Purpose

    Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-547 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated histidine 548 to a stop codon

    Promoter

    CMV

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-FLAG-Regnase-3_1-409 aa

    Plasmid

    #222658

    Purpose

    Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-409 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated proline 410 to a stop codon

    Promoter

    CMV

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-FLAG-Regnase-3_1-244 aa

    Plasmid

    #222659

    Purpose

    Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-244 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated asparagine 245 to a stop codon

    Promoter

    CMV

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

    Plasmid

    #222861

    Purpose

    This plasmid codes for the OgeuIscB nickase

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB Nickase
    

    Use

    CRISPR

    Tags

    3X HA and Nucleoplasmin NLS and ITR2

    Expression

    Mammalian

    Mutation

    E193A for nickase mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

    Plasmid

    #215898

    Purpose

    Expression of TNRC6A-CIM1 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

    Plasmid

    #215899

    Purpose

    Expression of TNRC6A-CIM2 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1529-1709

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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