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qTAG-N-Blast-V5

Plasmid

#207686

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTIR_Poetur_RFP

Plasmid

#248202

Purpose

Poetur RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF3084

Plasmid

#144560

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFP36L1 (ZFP36L1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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ReNL/pcDNA3

Plasmid

#85203

Purpose

Red color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Red enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G [N106/36] (Recombinant Antibody)

Antibody

#192039-rAb

Purpose

Anti-Ankyrin-G (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry and Immunohistochemistry

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits
qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC39A7_STOP

Plasmid

#161331

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A7 (SLC39A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

Plasmid

#223215

Purpose

Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

mCherry BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry (AAV Retrograde)

Viral Prep

#114470-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

EF1a

Tags

mCherry

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
ZJOM143

Plasmid

#133667

Purpose

Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJRA104

Plasmid

#209337

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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CyanoGate Kit

Plasmid Kit

#1000000146

Purpose

Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

Depositor

Alistair McCormick

Application

Cloning and Synthetic Biology

Vector Type

Bacterial Expression

Cloning Type

Golden Gate (MoClo)

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-V1

Pooled Library

#115643

Purpose

Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Has Service

Concentrated Lentiviral Prep

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only
PR_GEF YFP (2XPDZ-YFP-LARG DH)

Plasmid

#80408

Purpose

Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GRM4-Tango

Plasmid

#66389

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GRM4 (GRM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTRE-8XGTIIC-DsRED-DD

Plasmid

#115798

Purpose

lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

DsRED-DHFR

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-TIR-AAA

Plasmid

#187901

Purpose

Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

SQSTM1 (SQSTM1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Triple alanine mutant: P228A, E230A, P232A

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDESTmycDDX17

Plasmid

#19876

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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