161,273 results

1. EGFP-P2A-APEX2-ChBP

   Plasmid

   #108879

   Purpose

   Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   EGFP-P2A-APEX2-ChBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK-rABE-Flag

   Plasmid

   #191383

   Purpose

   Doxycycline inducible expression of RNA A-to-I editor rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   rABE-Flag
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   tet-inducible promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5aa SunTag VP64 EVD

   Plasmid

   #115483

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_C_TYRO3

   Plasmid

   #187793

   Purpose

   MAC-tagged gene expression of human TYRO3

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   TYRO3 (TYRO3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDMS151A_Tet-On-3G

   Plasmid

   #191577

   Purpose

   ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   Tet-On(R) 3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. V1-STING

   Plasmid

   #124263

   Purpose

   N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mArg1

   Plasmid

   #34573

   Depositor

   Peter Murray

   Insert

   Arg1 (Arg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK+ chickBMPR-1B CA

   Plasmid

   #49528

   Purpose

   cloning vector for constitutively active chick BMP receptor 1B

   Depositor

   Peter ten Dijke, Lee Niswander

   Article

   Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

   Insert

   BMPR-1B CA (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   constitutively active mutation (Q203D); also P2A,…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

9. p15A-OptoCre-knt-P-R*

   Plasmid

   #189852

   Purpose

   Cre-inducible activation of knt for kanamycin resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R*-knt
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P (T7 phage gene 10)

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDrVSP-IRES2-EGFP

    Plasmid

    #80333

    Purpose

    Expresses Danio rerio VSP with separated EGFP in mammalian cell

    Depositor

    Yasushi Okamura

    Article

    Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

    Insert

    Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. T/brachyury(2A-Dre)

    Plasmid

    #140761

    Purpose

    Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Dre
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Endogenous T/brachyury

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI NL4-3 Nef-HA-WT

    Plasmid

    #24162

    Depositor

    Warner Greene

    Article

    Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

    Insert

    Nef (nef HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

13. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 S727E (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN7-S15

    Plasmid

    #190665

    Purpose

    Expresses N7 ribosomal protein S15

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7 S15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVAX1-BMP2

    Plasmid

    #137909

    Purpose

    Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Insert

    BMP2 (BMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3basic luciferase and renilla_polyA

    Plasmid

    #128046

    Purpose

    pGL3 basic luciferase reporter with co-expression of renilla luciferase

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti puro HA-Ubiquitin

    Plasmid

    #74218

    Purpose

    Lentiviral expression of HA tagged Ubiquitin

    Depositor

    Melina Fan

    Insert

    ubiquitin (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Single ubiquitin coding sequence (full length UBB…

    Promoter

    CMV

    Available since

    March 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pMpGWB236

    Plasmid

    #68627

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLEX307-FURIN-puro

    Plasmid

    #158460

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRE-VEGFA EF1a-rtTA3

    Plasmid

    #195881

    Purpose

    Lentiviral construct of doxycycline inducible VEGFA

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    VEGFA (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-tdTomato

    Plasmid

    #159275

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

    Insert

    tdTomato
    

    Use

    AAV; S1 knockin donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223-HCK

    Plasmid

    #23879

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HCK (HCK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid

    #212965

    Purpose

    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    CasRx
    

    Use

    Transposon

    Tags

    P2A-Blasticidin resistance

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Fibronectin-human-plasma in pMAX

    Plasmid

    #120401

    Purpose

    enables eukaryotic expression of human plasma fibronectin

    Depositor

    Inaam Nakchbandi

    Article

    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

    Insert

    Fibronectin (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSecUAG_UTu1D

    Plasmid

    #233867

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant

    Depositor

    Dieter Söll, Natalie Krahn

    Inserts

    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG) and …

    Promoter

    EM7, araBAD, and native As SelD

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-cytoFLARE1.0-TEV

    Plasmid

    #234517

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    EGFP-P2A-CaM-V5-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge