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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRRL.gp91.EF1aIntron.eGFP

Plasmid

#30466

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/EF1aIntron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL.gp91.EF1aIntron.eGFP

Plasmid

#30466

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/EF1aIntron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Tags

1D4

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Tags

1D4

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-puroR-p2a-HA-halo

Plasmid

#161979

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

puroR-p2a-HA-halo-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge