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GST-FOXO3a WT

Plasmid

#1790

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2005

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inp54p

Plasmid

#96812

Purpose

cilia-membrane-targeted inositol polyphosphate 5-phosphatase

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inp54p

Tags

YFP

Expression

Mammalian

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1pr

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

Plasmid

#160044

Purpose

For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaH (iaaH )

Use

Synthetic Biology

Tags

3xNLS-Citrine

Expression

Mammalian

Promoter

pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 4

Plasmid

#188364

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pDNR-SpCas9

Plasmid

#80425

Purpose

Used for in vitro transcription of SpCas9 mRNA.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9

Use

CRISPR

Tags

none

Mutation

codon optimized for human

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-RT002-LV

Plasmid

#163382

Purpose

2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG17-VEGFRtm (LV)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF1A

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Smac(1-60)mCherry

Plasmid

#40880

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac(1-60)mCherry (DIABLO Human)

Tags

mCherry

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pCX-NTOM-SpiCee

Plasmid

#140902

Purpose

mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

NTOM-SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-Snail

Plasmid

#36976

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Snail (Snai1 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pHR_PGK_SNIPR_CD8alpha variant 2, G318A

Plasmid

#188365

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TetO-FUW-VdC9BV

Plasmid

#62195

Purpose

Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

Depositor

Kam Leong

Article

Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

Insert

VP64dCas9BFPVP64

Use

Lentiviral

Tags

Two VP64s tagged to dCas9 fused to BFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive) Cas9 (dCas9)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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