We narrowed to 18,492 results for: REV

1. pCfB2798

   Plasmid

   #63659

   Purpose

   pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSW149

   Plasmid

   #122248

   Purpose

   pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

   Depositor

   Sarah Walker

   Article

   Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

   Insert

   TIF4631 (TIF4631 Budding Yeast)
   

   Tags

   Intein-Chitin-binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p403-BrdU-Inc

   Plasmid

   #71789

   Purpose

   BrdU-incorporating plasmid with selectable marker HIS3

   Depositor

   Oscar Aparicio

   Article

   Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

   Inserts

   Thymidine Kinase
   ENT1 (SLC29A1 Human)
   

   Expression

   Yeast

   Promoter

   ADH1 and GPD

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO mouse shRNA 1 raptor

   Plasmid

   #21339

   Depositor

   David Sabatini

   Article

   Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

   Insert

   raptor shRNA 1 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 7, 2009

   Availability

   Academic Institutions and Nonprofits only

5. GW1-rogRFP2

   Plasmid

   #207876

   Purpose

   Mammalian expression of the red fluorescent rogRFP2 redox sensor

   Depositor

   Mathew Tantama

   Article

   Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

   Insert

   rogRFP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEK36

   Plasmid

   #210598

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
   

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Promoter

   SpH2Bpro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LbdCas12a VPR

   Plasmid

   #188513

   Purpose

   Expresses FLAG tagged LbdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-GFP (F64L, S65T, V163A)-TRP1

   Plasmid

   #53184

   Purpose

   chromosomal tagging with stable GFP

   Depositor

   John Pringle

   Article

   Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A)

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pKP3915

   Plasmid

   #113335

   Purpose

   Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

   Insert

   Artificial Transcription Factor (McIsaac et al, 2013)
   

   Expression

   Yeast

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAT9624-BEAR-cloning

    Plasmid

    #162986

    Purpose

    BEAR target cloning plasmid with split EGFP

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGI3EM22C

    Plasmid

    #135488

    Purpose

    Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr LifeAct-tdTomato
    

    Tags

    LifeAct

    Expression

    Bacterial and Yeast

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJT9R

    Plasmid

    #98305

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBa.GFP-KIF13B

    Plasmid

    #134626

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-FLAG-APEX2-OPTN

    Plasmid

    #175760

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-hGFAP-NEUROD1-T2A-mCherry

    Plasmid

    #178582

    Purpose

    Expression of NEUROD1 and mCherry under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-link-GFPEnvy-SpHis5

    Plasmid

    #60782

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPEnvy
    

    Use

    Yeast integrating

    Mutation

    S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-4T HHARI RBR

    Plasmid

    #31254

    Depositor

    Rachel Klevit

    Article

    Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

    Insert

    Human Homologue of Ariadne (HHARI) (ARIH1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    RBR domain residues 177-395

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pMXs-NDUFAF5-HA

    Plasmid

    #86125

    Purpose

    Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    NDUFAF5 (NDUFAF5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Mucin MUC1-5TR-pET15b

    Plasmid

    #174694

    Purpose

    Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

    Depositor

    Jean Gariepy

    Article

    Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

    Insert

    MUC1-TR
    

    Tags

    hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV8-cPE-N

    Plasmid

    #183538

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    N-terminal fragment of SpCas9 nickase (H840A)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Tags

    yomRuby3

    Expression

    Yeast

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEK22

    Plasmid

    #210588

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    BdH3pro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Promoter

    BdH3pro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge