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pQCXIZ GFP PODXL 5N>Q

Plasmid

#202419

Purpose

Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

N33/43/104/144/360Q

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD8_STOP

Plasmid

#161048

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD8 (MFSD8 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_CDC42_WT

Plasmid

#82104

Purpose

Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDC42 (CDC42 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD14B_STOP

Plasmid

#161047

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD14B (HIATL1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

R505C

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-DIRC2_STOP

Plasmid

#161046

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

DIRC2 (DIRC2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-AKT1-R121W

Plasmid

#116104

Purpose

Lentiviral expression of AKT1 R121W

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Mutation

R121W

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MPZL1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156511

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MPZL1 (MPZL1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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shYAP1 # 1

Plasmid

#42540

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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shYAP1 # 2

Plasmid

#42541

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1

Plasmid

#213583

Purpose

Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG-YAP1

Plasmid

#66853

Purpose

Express FLAG-tagged YAP1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

YAP1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2258

Plasmid

#142927

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX11 (SOX11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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R701-X65-527: His6-tev-Hs.CALM1(2-148)

Plasmid

#159693

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(2-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(2-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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hnRNPA1_D262V-FLAG-IRES-eGFP

Plasmid

#234620

Purpose

Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 D262V (HNRNPA1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Famillial ALS mutation D262V, C-terminal FLAG tag…

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WKKD

Plasmid

#111905

Purpose

Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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