We narrowed to 80,379 results for: TRI

1. SPHKAP scFv [L131/17]

   Plasmid

   #190511

   Purpose

   Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES2-EGFP-deltaNp53:WTp53

   Plasmid

   #49244

   Purpose

   expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

   Depositor

   Dylan Taatjes

   Article

   Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

   Insert

   p53 deltaN and p53 WT (TP53 Human)
   

   Use

   Tags

   His and IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pThermoCas9_ctrl

   Plasmid

   #100981

   Purpose

   Expresses ThermoCas9 and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Parvalbumin scFv [L114/38]

   Plasmid

   #206777

   Purpose

   Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENTICRISPR-ALDOA_sgRNA1

   Plasmid

   #201590

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   ALDOA (ALDOA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pan-Synapsin scFv [L125/129]

   Plasmid

   #182079

   Purpose

   Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting pan-Synapsin
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcBac1-Mb-sfGFP WT

   Plasmid

   #197569

   Purpose

   Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP WT
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hHSF1 RNAi resistant

   Plasmid

   #118347

   Purpose

   This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   RNAi resistance mutations (does not change amino …

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-Hsp90 N(9-236)

   Plasmid

   #22481

   Depositor

   William Sessa

   Article

   Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

   Insert

   Hsp90 alpha N domain (HSP90AA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Con/Foff 2.0-EYFP

    Plasmid

    #137162

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINB-hRNF216(457-784)

    Plasmid

    #171922

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 MLL2 653

    Plasmid

    #11017

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MLL2 (KMT2B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    truncation of MLL2, last 653 amino acids, from pr…

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Con/Fon/Von eYFP

    Plasmid

    #137164

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV Retrograde and AAV8

    Insert

    3x-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Fon-iC++-EYFP

    Plasmid

    #137155

    Purpose

    Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+FLAG-KrasnatG12D

    Plasmid

    #206842

    Purpose

    To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Aspartic Acid

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BII-BCA-Prom-ASCL2

    Plasmid

    #133394

    Purpose

    Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    destabilized tdTomato-NLS and destabilized NLS-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free) drives expressi…

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GC52 pEGFPN1 anti-Gephyrin nanobody

    Plasmid

    #135224

    Purpose

    Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

    Plasmid

    #170195

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    Nov. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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