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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,905 results for: sta

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Showing: 6341 - 6360 of 14905 results

  6. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Promoter
    -
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124802-plasmid-map-sequence-id-244317

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  8. pKK-RNAi-nucEGFPmiR-mCherry-TEV

    Plasmid
    #105808
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    mCherry-TEV
    Expression
    Mammalian
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105808-plasmid-map-sequence-id-274623

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  9. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid
    #105812
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105812-plasmid-map-sequence-id-203423

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  10. pHyg-PCUP1-AID*(N)

    Plasmid
    #99529
    Purpose
    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Insert
    IAA17(71-117) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Promoter
    CUP1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99529-plasmid-map-sequence-id-192013

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  14. pcDNA-Dest47- WTN23C11orf83-GFP

    Plasmid
    #65845
    Purpose
    Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)
    Depositor
    Amos Bairoch
    Publication
    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    WT Transmembrane
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65845-plasmid-map-sequence-id-112008

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  15. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Publication
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  19. pB-3xFlag-dCas13-mTET2CD

    Plasmid
    #228234
    Purpose
    For targeted tethering of the catalytic domain of mouse TET2 using dCas13
    Depositor
    Chuan He
    Publication
    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas13
    TET2 (Tet2 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    CD (cysteine-rich, dioxygenase domain) truncation…
    Promoter
    CAG
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228234-plasmid-map-sequence-id-451264

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  20. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Publication
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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Showing: 6341 - 6360 of 14905 results