We narrowed to 17,342 results for: She

1. Phat2-hs-cPla2-C2-EGFP-GH

   Plasmid

   #187088

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000116711 (PLA2G4A Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FusionRed-C-Src-7

   Plasmid

   #56107

   Purpose

   Localization: Membrane/Vesicles, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   C-Src
   

   Use

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pTORKm43GWC

   Plasmid

   #112898

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)-Rab10-FLAG

   Plasmid

   #135559

   Purpose

   Express mouse Rab10 in mammalian cells, FLAG at N terminal

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CBP-HAT-pET

   Plasmid

   #21091

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   HAT domain aa 1171-1621

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

6. lentiCRISPR-v2-FE-PuroR

   Plasmid

   #186746

   Purpose

   Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTORKm43GWY

   Plasmid

   #112901

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.11 promPDE4D3 Ebox mut

    Plasmid

    #27304

    Depositor

    David E. Fisher

    Article

    Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

    Insert

    prom PDE4D3 (PDE4D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Ebox CACATG changed to GAGATG

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Beta-arrestin2 K11,12R GFP

    Plasmid

    #35412

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K11,12R

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HOXB6-pCMV

    Plasmid

    #21258

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Tags

    FLAG_Pro Tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pEBB-3XMyc-TRAF2 S11A

    Plasmid

    #44105

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S11A (TRAF2 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Serine 11 to Alanine

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLdSaCN

    Plasmid

    #123261

    Purpose

    Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Insert

    gRNA and SaCas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FusionRed-BaxAlpha-C-15

    Plasmid

    #56101

    Purpose

    Localization: , Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    BaxAlpha
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. PB-HD118

    Plasmid

    #172655

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD118A/pegRNA-HD118B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CBP-Bromo-SP65

    Plasmid

    #21089

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    Bromo domain aa 1107-1172

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pKT-SCFP3A-HIS

    Plasmid

    #64686

    Purpose

    pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    SCFP3A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pXJ40 GADD34 (214-513aa)

    Plasmid

    #78095

    Purpose

    mammalian expression of GADD34 214-513 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 214-513

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pKT-mCitrineV163A-HIS

    Plasmid

    #64685

    Purpose

    pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    mCitrine V163A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Valine 163 changed to alanine

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CBP-HAT-SP65

    Plasmid

    #21088

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    HAT domain aa 1172-1621

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

26. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

27. XE93 mFZ3-CS2+

    Plasmid

    #16804

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    mFZ3-CS2+ (Fzd3 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pLX-V5-TRAF2 S11A-puro

    Plasmid

    #44112

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S11A (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Serine 11 to Alanine

    Promoter

    Available since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

29. CBP-CH3-SP65

    Plasmid

    #21092

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    In vitro tnt

    Tags

    Expression

    Mutation

    CH3 domain aa 1625-1877

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pLX-V5-TRAF2 S102A-puro

    Plasmid

    #44113

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S102A (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Serine 102 to Alanine

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

31. FusionRed-CytERM-N-17

    Plasmid

    #56110

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CytERM
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. XE107 XPKC alpha-GFP-CS2+

    Plasmid

    #16743

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    PKC-alpha (prkca Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

33. XE96 mFZ7-CS2+

    Plasmid

    #16801

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    FZ7 (Fzd7 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

34. CBP-Bromo-HAT-SP65

    Plasmid

    #21090

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    Bromo+HAT domains aa 1107-1621

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSCKtheoRaj12m

    Plasmid

    #69943

    Purpose

    pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    pSCKtheoRaj12m
    

    Use

    Synthetic Biology; Psc101 origin of replication

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FusionRed-CAF1-C-10

    Plasmid

    #56102

    Purpose

    Localization: Nucleus, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CAF1
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pU6_(GLuc)_INT[GFPApt]

    Plasmid

    #68428

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the GFP aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pU6_(GLuc)_INT[3xK-T]

    Plasmid

    #68429

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three kink-turns
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. XW83

    Plasmid

    #65841

    Purpose

    MosSCI-Pmig-13::QF::SL2::mCherry

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge