Addgene

We narrowed to 11,162 results for: ENA

Showing: 1551 - 1600 of 11162 results

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pAC573

Plasmid

#127974

Purpose

AMA1 plasmid with argB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

argB

Use

Fungal expression

Tags

Expression

Bacterial

Mutation

Promoter

native promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-17-6

Plasmid

#190827

Purpose

For mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

17-6 (Non-aptamer RNA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pE1B-C-F/Sox10-MCS1

Plasmid

#20238

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pRL2697

Plasmid

#70693

Purpose

pDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinker

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS1C

Plasmid

#20240

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS1C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[V5A2G3-60]

Plasmid

#67009

Purpose

Expresses malE-ELP[V5A2G3-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V5A2G3-60]

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS8

Plasmid

#20247

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Apr)

Plasmid

#110091

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltasacB (Kan)

Plasmid

#110092

Purpose

Analogous to pKO but without the negative selection gene sacB.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHCKAn-yibDp-GRFT

Plasmid

#158745

Purpose

low phosphate yibDp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

yibDp Griffithsin

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibD

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS1B

Plasmid

#20239

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS1B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS9

Plasmid

#20248

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS2

Plasmid

#20241

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS6

Plasmid

#20245

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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SF-1 shRNA #1

Plasmid

#28186

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pE1B-C-F/Sox10-MCS3

Plasmid

#20242

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-31 site

Plasmid

#25021

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-31 site

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSDH51

Plasmid

#139045

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSM10

Plasmid

#139034

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mScarlet (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM05

Plasmid

#139050

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp9-3

Plasmid

#73854

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
SPL10p::pCGTAG (SPL10p::NLS-GFP)

Plasmid

#27410

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 promoter (AT1G27370 Mustard Weed)

Use

Tags

Expression

Mutation

SPL10 promoter transcriptionally fused to a nucle…

Promoter

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pAWp9-1

Plasmid

#73852

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-GFP-sg1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #2

Plasmid

#28187

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #3

Plasmid

#28188

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pXD80EN13-Elen0616-Cber(ElDSM2243)

Plasmid

#225308

Purpose

Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cber

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Use

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1462

Plasmid

#218623

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Use

Tags

ePTS1

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1463

Plasmid

#218624

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Use

Tags

ePTS1

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1734

Plasmid

#218627

Purpose

Express amorphadiene synthase (ADS)

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

ADS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1535

Plasmid

#218613

Purpose

Express Pex5

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1536

Plasmid

#218617

Purpose

Express Pex5 and Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1376

Plasmid

#218618

Purpose

Express Pex5, Pex3, Pex19, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1577

Plasmid

#218619

Purpose

Express Pex5, Pex2, Pex17, and Pex10

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1338

Plasmid

#218592

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Use

Tags

Expression

Yeast

Mutation

Pex22(1-36)

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB207

Plasmid

#218596

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Use

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GSPD1_R252L

Plasmid

#224153

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(R252L) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(R252L)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

R252L

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GSPD1_AJ

Plasmid

#224152

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1 with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1 (B0035.5 Nematode)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GSPD1_D60N

Plasmid

#224154

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(D60N) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(D60N)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

D60N

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN008

Plasmid

#220049

Purpose

Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

flag-HA-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN053

Plasmid

#220047

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVTHM-miniSOX-3xflag

Plasmid

#206378

Purpose

Expresses 3xflag fused mouse miniSOX in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

miniSOX

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-MTS-jAspSnFR3

Plasmid

#203487

Purpose

Mitochondrial directed jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Lentiviral

Tags

Mitochondrial localization signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-CAG-jAspSnFR3-mRuby3

Plasmid

#203500

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA791

Plasmid

#186246

Purpose

dnap-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA792

Plasmid

#186247

Purpose

dnap-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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