We narrowed to 24,089 results for: Spr

1. pWS5395-CRISPRai-LYS2

   Plasmid

   #182834

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS5391-CRISPRai-LEU2

   Plasmid

   #182830

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2-Blast-mU6

   Plasmid

   #206806

   Purpose

   pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

   Depositor

   Jolanda Van Leeuwen

   Article

   Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. R777-E333 Hs.SPRY4

   Plasmid

   #70618

   Purpose

   Gateway ORF clone of human SPRY4 [NM_001127496.1] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SPRY4 (SPRY4 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZR064_CROPseq-sgCD4-CRISPRi

   Plasmid

   #180270

   Purpose

   Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   CD4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR - EGFP sgRNA 6

   Plasmid

   #51765

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 6
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-CRISPR-v2-gYap-1

   Plasmid

   #166481

   Purpose

   Expresses spCas9 and a gRNA targeting mouse Yap

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   gRNA for mouse Yap (Yap1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2-sgLmnb2-HepmCherry

   Plasmid

   #192830

   Purpose

   Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2-Stuffer-HepmTurquoise2

   Plasmid

   #192826

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mTurquoise2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAf-CRISPR-phoA

    Plasmid

    #207991

    Purpose

    CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    phoA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-CRISPR-V2-gYap-2

    Plasmid

    #166482

    Purpose

    Expresses spCas9 and a gRNA targeting mouse Yap

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    gRNA for mouse Yap (Yap1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E325 Hs.SPRED3

    Plasmid

    #70609

    Purpose

    Gateway ORF clone of human SPRED3 [NM_001042522.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRED3 (SPRED3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. lentiCRISPR-v2-Blast-Puro

    Plasmid

    #167186

    Purpose

    Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CRISPR sgSHMT2_1

    Plasmid

    #106308

    Purpose

    Express Cas9 and sgRNA targeting SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-Crispr-V2-HMID.v1

    Plasmid

    #103062

    Purpose

    LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    Cas9 plus expressed GESTALT V1-V5 guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CRISPR sgSHMT1_1

    Plasmid

    #106311

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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