165,174 results
-
Plasmid#179396PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromoDepositorInsertCBX7_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationG113V in flexible linkerPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1665
Plasmid#141863PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP73 (TP73 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R273H
Plasmid#16439DepositorInsertp53 R273H (TP53 Human)
UseTagsExpressionMammalianMutationR273HPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
IL13 promoter luciferase
Plasmid#11784DepositorInsertIL-13 promoter (IL13 Human)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Annexin-12
Plasmid#55276DepositorInsertAnnexin (A4) (ANXA4 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_PA-Cre
Plasmid#122960PurposePhotoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)DepositorInsertpcDNA3.1_PA-Cre
UseCre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-UbxD8
Plasmid#53777Purposeexpress FLAG-tagged human UbxD8 geneDepositorInsertUbxD8 (FAF2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pXG237
Plasmid#141281PurposeATF4 translational reporterDepositorInsertuORF1/2 of ATF4 followed by mApple
UseLentiviralTagsmAppleExpressionMutationPromoterCMVAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga11
Plasmid#196059PurposeEncodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas9
Plasmid#124890Purposeobtaining full Cas9 RNPDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-HIF1B (#930)
Plasmid#99916Purposemammalian expressionDepositorInsertHIF1B (ARNT Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis
Plasmid#157024PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertJAG1 (JAG1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-COPS5
Plasmid#22541DepositorInsertCOPS5 (COPS5 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…TagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorInsertMutL homolog 1 (MLH1 Human)
UseLentiviralTagsExpressionMutationΔ754-756 dominant negative mutantPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5DN(S34N)
Plasmid#35139DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationDominant Negative- S34NPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
TGT-His
Plasmid#138201PurposeExpresses E. Coli TGT with a C-terminal His TagDepositorInsertE Coli tRNA Guanine Transglycosylase-His
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP22
Plasmid#22575DepositorInsertUSP22 (USP22 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTNS3
Plasmid#63127PurposeTn7 transposase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterP1 and PtacAvailable sinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5-Smad7-HA
Plasmid#11733DepositorInsertSMAD7 (SMAD7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
Plasmid#86157PurposeFLS2-GFP expresssionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTags3xmyc and GFPExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SUPERBLADE5
Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPSeq-BFP
Plasmid#196718PurposeCROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only