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pKrox24(MapErk)Luc

Plasmid

#200112

Purpose

Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence in minimal p…

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-4mtGCaMP6f-DIO

Plasmid

#179529

Purpose

Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Has Service

AAV1

Insert

4mtGCaMP6f

Use

AAV

Tags

4x Cox8 targeting sequence

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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σ2-mCherry

Plasmid

#186579

Purpose

Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP2S1 (AP2S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MSI2-DCD

Plasmid

#166968

Purpose

Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-DCD

Use

Retroviral

Tags

MSI2-DCD

Expression

Mammalian

Mutation

N495S

Promoter

MSCV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-RUNX1

Plasmid

#139821

Purpose

doxycycline-inducible expression of Human RUNX1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Runx1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP and His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP(V5-APEX2-NES)

Plasmid

#107596

Purpose

Expresses V5-APEX2-NES in mammalian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

APEX2-NES

Use

Frt site to insert into hek293 t-rex genome

Tags

NES and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-mito-roGFP1-mApple

Plasmid

#107218

Purpose

Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

roGFP1-mApple

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_alphaTubulin_C1

Plasmid

#85046

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-Ecadherin

Plasmid

#65291

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Expression

Mammalian

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
Blue opsin mcherry ---- pcDNA3.1

Plasmid

#62932

Purpose

Blue opsin tagged with mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

blue opsin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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