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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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169,450 results

Showing: 6461 - 6480 of 169450 results

  5. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid
    #135281
    Purpose
    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-CDK12 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135281-plasmid-map-sequence-id-267208

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  6. MTNR1A-DuET

    Plasmid
    #213348
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MTNR1A (MTNR1A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213348-plasmid-map-sequence-id-430874

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  8. INCENP

    Plasmid
    #39185
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INCENP (INCENP Human)
    Tags
    GST-Tev
    Expression
    Bacterial
    Mutation
    Complex with AURKBA-c004
    Available Since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pHJ4: LexA-VHH-Caffeine

    Plasmid
    #108242
    Purpose
    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    LexA DNA binding domain fusing with VHH-Caffeine antibody
    Use
    Synthetic Biology
    Promoter
    pLacO1
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    108242-plasmid-map-sequence-id-212131

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  12. TFORF0656

    Plasmid
    #141653
    Purpose
    Lentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CHD3 (CHD3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141653-plasmid-map-sequence-id-387044

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  16. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid Kit
    #1000000159
    Purpose
    The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.
    Depositor
    Vladimir Nekrasov
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 6461 - 6480 of 169450 results