165,185 results

1. pEJS1096 Dual-sgRNA.Design 1

   Plasmid

   #159538

   Purpose

   Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 nuclease with two guide RNA cassettes with promoters
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0079 RESCUE-S

   Plasmid

   #130662

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETduet+sfGFP-TetR-p300HAT

   Plasmid

   #157795

   Purpose

   Expression of sfGFP-TetR-p300HAT fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   sfGFP-TetR-p300 (EP300 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

   Plasmid

   #126480

   Purpose

   Expresses Cas9-GSAlinker-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   GSA linker
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB505

   Plasmid

   #74847

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL056

   Plasmid

   #177168

   Purpose

   Expresses wildtype MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1-gfaABC1D-PinkyCaMP

   Plasmid

   #232859

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Beta-Globin-WT

   Plasmid

   #130700

   Purpose

   Expresses Beta-Globin WT NMD reporter

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   Beta Globin (HBB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBIS

   Plasmid

   #17817

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-Flpo

    Plasmid

    #55637

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV2

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgYAP-TAZ-3

    Plasmid

    #229453

    Purpose

    knockout of YAP1 and WWTR1 (TAZ)

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-TAZ-3 (YAP1, WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1A-CD19.28H.28TM.28.3z

    Plasmid

    #200678

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1-SypHer3s-IMS

    Plasmid

    #108120

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-IMS
    

    Use

    Tags

    ratiometricYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFN43

    Plasmid

    #89487

    Purpose

    gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

    Depositor

    Alejandro Ferrando

    Article

    Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    YFP-N (aa 1-154)

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E-mpeg1.1

    Plasmid

    #75023

    Purpose

    Can be used to drive expression in zebrafish microglia.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHIV-Zsgreen

    Plasmid

    #18121

    Depositor

    Bryan Welm, Zena Werb

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    Zsgreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD8a-EGFP

    Plasmid

    #86051

    Purpose

    A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SPLICSFAST-LDh-LY-P2A

    Plasmid

    #214895

    Purpose

    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LDh-LY-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC-tag-N

    Plasmid

    #108078

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMV-pDisplay-SNAP

    Plasmid

    #223495

    Purpose

    Expression of SNAP-tags to outer surface in mammalian cells

    Depositor

    James Frank

    Article

    Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

    Insert

    SNAP-tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHIV-INK4A-zsGreen

    Plasmid

    #110728

    Purpose

    Mammalian Expression of p16INK4A IRES zsGreen

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p16INK4A IRES zsGreen (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOPTN-EGFP

    Plasmid

    #27052

    Depositor

    Beatrice Yue

    Article

    Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MYH11-NanoLuc-2A-tdTomato

    Plasmid

    #132569

    Purpose

    Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    Human MYH11 knockin homology arms (MYH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-Tau(T217E)

    Plasmid

    #187024

    Purpose

    Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 217 to Glutamic acid

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Use

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MX1_pCSdest

    Plasmid

    #53951

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    MX1 (MX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. EphA2-MT

    Plasmid

    #108851

    Purpose

    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Tags

    Labeled with mTurquoise on the C-Terminus via a 1…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAd/CMV/V5-Clasp2α

    Plasmid

    #89763

    Purpose

    Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CLASP2α (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge