We narrowed to 165,741 results for: DES
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Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH
Plasmid#100049PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activationDepositorInserthChR2(E123T/H134R)
UseAAVTagsEYFPExpressionMammalianMutationhumanized, E123T/H134RPromoterhSynapAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-URA3
Plasmid#168059PurposeReplaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymNeonGreen
TagsymNeonGreenExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBG-bZIPa
Plasmid#12436DepositorInsertbZIP of C/EBPa (CEBPA Human)
TagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133
Plasmid#164822PurposeLentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic NotchDepositorInsertsAntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
UseLentiviralTagsmCherryPromoterminiCMV and pGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorAvailable SinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc2
Plasmid#134451PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralExpressionMammalianPromoterCMV enhancer/chicken beta actinAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCX-HO1-2A-EGFP
Plasmid#74672PurposeMammalian expression of HO1 and EGFPDepositorAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Cdc42-2G
Plasmid#68812PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)DepositorInsertCdc42 activation reporter
UseAdenoviralTagsHis and MycExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP-R270X
Plasmid#48079PurposeMammalian expression of GFP tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
TagsGFPExpressionMammalianMutationexpresses AT-Hook R270XPromoterCMVAvailable SinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
TurboID-mNeongreen-3xFLAG fusion pos3
Plasmid#172864PurposeC. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion proteinDepositorInsertTurboID
UseUnspecifiedTagsFLAG, mNeongreen, and mycAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSDest-MYC-TMPRSS2
Plasmid#154964PurposeTMPRSS2 with N-terminal MYCDepositorAvailable SinceJuly 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…ExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianPromoterCMV IE94 promoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
Plasmid#124549PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)DepositorInsertp65 promoter- p65 CDS-Clover
UseLentiviralTagsCloverExpressionMammalianAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Strep2
Plasmid#119753PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-ANLN
Plasmid#31203DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS
Plasmid#158741PurposeExpresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFPDepositorInsertHemagglutinin A/MI/45/15 H1N1
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationAA61-469PromoterCMVAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-KIF5B*-strep
Plasmid#120170PurposeExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
TagsHA and StreptavidinExpressionMammalianMutationTruncated KIF5B: 1-555PromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AEtr
Plasmid#12432DepositorAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher
Plasmid#216284PurposeExpresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked bindersDepositorInsertDoubleCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only