We narrowed to 18,589 results for: MUT

1. pET-28a(+)-FGF1-4X

   Plasmid

   #120282

   Purpose

   Produce human recombinant FGF1-4X in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 1 mutant
   

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAN057

   Plasmid

   #220053

   Purpose

   NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
   

   Expression

   Mammalian

   Mutation

   c.3846G>A mutation

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRR.1

   Plasmid

   #69995

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGB_YFP-MiniTurboID

   Plasmid

   #222433

   Purpose

   Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLAG-Egfr(Velvet)

   Plasmid

   #18788

   Depositor

   Bruce Beutler

   Article

   Du et al Genetics. 2004 Jan . 166(1):331-40.

   Insert

   epidermal growth factor receptor (Egfr Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D833G; signal sequence removed (aa1-24)

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. OPEN-HLA-A*11:01-BirA

   Plasmid

   #235135

   Purpose

   E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*11:01 (HLA-A Human)
   

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1_SPTLC1_WT

   Plasmid

   #181920

   Purpose

   3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

   Depositor

   Christian Metallo

   Article

   Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

   Insert

   Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
   

   Use

   Lentiviral; Mammalian expression, , doxycycline i…

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-HA-hRb-delta-CDK-puro

    Plasmid

    #212673

    Purpose

    Express tagged hRb delta-CDK phosphosite mutant

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    All 15 CDK phospho-sites are mutated to alanines

    Promoter

    TRE

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

    Plasmid

    #86851

    Purpose

    mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP6 (ATP6 Human)
    

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon corrected

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6-DEST PINK1-V5 KD

    Plasmid

    #13319

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Kinase dead: K219A/D362A/D384A

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG491

    Plasmid

    #226747

    Purpose

    CRISPRi guide RNA against MTCH2 (dual guide)

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid

    #127354

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF152 pcDNA3.1(+)SOD1A4V

    Plasmid

    #26398

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Mutation

    A4V

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-K-GECO1

    Plasmid

    #105864

    Purpose

    Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Tags

    6-His Tag

    Expression

    Bacterial

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. V2R-GFP

    Plasmid

    #208910

    Purpose

    encodes the human vasopressin receptor 2, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

    Insert

    AVPR2 (AVPR2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-Sec24D

    Plasmid

    #32677

    Depositor

    Henry Lester

    Article

    Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

    Insert

    Sec24D (SEC24D Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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