We narrowed to 29,498 results for: Ret;

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RabV CVS-N2c(deltaG)-Cre

Plasmid

#73474

Purpose

Expresses Cre for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

Cre recombinase

Use

Neurotropic virus

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJM010

Plasmid

#134154

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PM

Plasmid

#197386

Purpose

To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTub-Wg

Plasmid

#64321

Purpose

Expresses Drosophila Wingless protein under the tubulin promotor

Depositor

Roel Nusse

Article

Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

Insert

Wingless (wg Fly)

Use

Drosophila expression

Promoter

alpha-1 tubulin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG mCherry-LDTS-eLIR

Plasmid

#189002

Purpose

lipophagy-inducing adaptor protein, AAV

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-eLIR, mCherry

Use

AAV

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2

Plasmid

#13801

Depositor

Roger Davis

Article

Sluss et al Mol Cell Biol. 1994 Dec . 14(12):8376-84

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pSuper mouse Dicer1

Plasmid

#14779

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pRV∆L-5Cre

Plasmid

#182964

Purpose

The 3rd generation of rabies vector expressing Cre recombinase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

CRE recombinase

Use

Rabies

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

HA

Expression

Mammalian

Promoter

Tet-responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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mNG-sfTq2ox

Plasmid

#124219

Purpose

Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-sfTq2ox

Expression

Mammalian

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pTEI036

Plasmid

#105350

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

LexA operator & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 1x (S1x)

Plasmid

#37003

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

One copy of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-subtilase A

Plasmid

#35948

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

Subtilase A (subA E. Coli)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only