We narrowed to 66,569 results for: des.1

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSP1673

Plasmid

#65769

Purpose

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-SUMO2-Q90P

Plasmid

#36448

Purpose

Expresses HA-Sumo2Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutated Q90P

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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plv-AcGFP-SOD1 (G93A)

Plasmid

#27142

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

AcGFP

Mutation

G93A

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-QF

Plasmid

#24339

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Tags

His and Myc

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pJJDuet30

Plasmid

#11962

Depositor

Hideo Iwai

Article

Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

Insert

Ssp DnaE-N

Tags

GB1

Expression

Bacterial

Available Since

Sept. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3B

Plasmid

#13743

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FIH1

Plasmid

#21403

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pGL3 GATA Luc

Plasmid

#85695

Purpose

Luciferase reporter containing three repeats of the GATA consensus site

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

3xGATA and minimal MT promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

3xGATA and minimal MT promoter

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pLKO-1: shALAS-1

Plasmid

#22750

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
EMSV-Myf5 (puro)

Plasmid

#14711

Depositor

Michael Rudnicki

Article

Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

Insert

Myf5 (Myf5 Mouse)

Expression

Mammalian

Available Since

April 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

Plasmid

#181980

Purpose

Human RUNX1C gene overexpression

Depositor

Jan-Henning Klusmann

Article

Gialesaki et al Blood. 2023 Mar 9;141(10):1105-1118. doi

Insert

RUNX1C (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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PU.1-delta75-100 LZRS

Plasmid

#44634

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 Δ75-100 (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

deletion of aa 75-100

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pMaster1

Plasmid

#58525

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES

Promoter

CAG and EF1a

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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