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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 786 results for: gc

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Showing: 641 - 660 of 786 results

  12. pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA

    Plasmid
    #222404
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.
    Depositor
    Katherine Berry
    Article
    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)
    Insert
    5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
    Tags
    1xMS2 hairpin, 7GC clamp, TtrpA terminator
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    222404-plasmid-map-sequence-id-451848

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  16. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  19. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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Showing: 641 - 660 of 786 results