We narrowed to 9,277 results for: Pol;

1. WT USP7 UBL-12

   Plasmid

   #227230

   Purpose

   Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 535-775

   Promoter

   T7-promoter

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-WzxE-TEV-His10

   Plasmid

   #226781

   Purpose

   Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

   Depositor

   James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE
   

   Tags

   TEV cleavable 10xHistidine tag

   Expression

   Bacterial

   Mutation

   valine insertion in position 2 due to the cloning…

   Promoter

   araBAD

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

   Plasmid

   #227103

   Purpose

   DRD2 receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   DRD2-NTEV (DRD2 Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG8Ha-His-EWSR1-359-549

   Plasmid

   #195868

   Purpose

   Expresses the RRM-RG2-ZnF (359-549) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWS 359-549 (EWSR1 Human)
   

   Tags

   His8-linker-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK7190

   Plasmid

   #219763

   Purpose

   MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   nnLuz, nnH3H, nnCPH
   

   Expression

   Plant

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX459v3-eSpCas9(1.1)

   Plasmid

   #178800

   Purpose

   High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX459v3-SpCas9-HF1

   Plasmid

   #178801

   Purpose

   High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK189

   Plasmid

   #219743

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Mutation

   D37E, V181I, S323M, M385K

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK4169

   Plasmid

   #219744

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Mutation

   D37E, V181I, S323M, M385K

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNK7183

    Plasmid

    #219760

    Purpose

    MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PzPKS2
    

    Expression

    Plant

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNK7180

    Plasmid

    #219762

    Purpose

    MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    HmS
    

    Expression

    Plant

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-EGFR*-CUbo-His6

    Plasmid

    #212819

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Tags

    CUbo-6xHis

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

    Plasmid

    #212822

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Tags

    CUbo(K63R)

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

    Plasmid

    #201576

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A, M173A and V195A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)

    Plasmid

    #201460

    Purpose

    Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks N-terminal residues 1-110

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)

    Plasmid

    #201463

    Purpose

    Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 161-201

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)

    Plasmid

    #201465

    Purpose

    Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks C-terminal residues 318-363

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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