We narrowed to 10,386 results for: Coli
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Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
TagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pROS12
Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS15
Plasmid#107929Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3a_Ub1
Plasmid#156467PurposeBacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tagDepositorInsertNsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pROS14
Plasmid#107928PurposeLEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAD54_CAD29_H104A_pvp008
Plasmid#124876PurposeExpresses hCAD His103Ala mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated His103 to Ala, deleted amino acids 1-3 an…PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-29Q
Plasmid#84350PurposeExpression of the human Huntingtin Exon1 protein containing 29Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-15Q
Plasmid#84348PurposeExpression of the human Huntingtin Exon1 protein containing 15Q in E.coliDepositorInsertHuntingtin Exon1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMG035 MBP-Axin∆BCD-His
Plasmid#154053PurposeExpresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM2-mRUBY2
Plasmid#107793Purposedonor vector for knock-in gene editing at human DNM2 locusDepositorInsertDNM2-mRuby2 (DNM2 Human, Synthetic)
UseYeast-e.coli shuttle vectorExpressionBacterial and YeastAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAD96_hIRG1_4-461_pvp008
Plasmid#124874PurposeExpresses hCAD Asn152Ser mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated Asn152 to Ser, deleted amino acids 1-3 an…PromoterT7Available SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-N
Plasmid#156469PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-M
Plasmid#156470PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core_Q360L
Plasmid#233619PurposeExpression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coliDepositorInsertDDX3X helicase core Gln360Leu (DDX3X Human)
TagsHis6ExpressionBacterialMutationGln360LeuAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core_Q360P
Plasmid#233620PurposeExpression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coliDepositorInsertDDX3X helicase core Gln360Pro (DDX3X Human)
TagsHis6ExpressionBacterialMutationGln360ProAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1 M5
Plasmid#204563PurposeExpresses GST-tagged UFBP1 M5 in E.coliDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1 M3
Plasmid#204561PurposeExpresses GST-tagged UFBP1 M3 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1 M4
Plasmid#204562PurposeExpresses GST-tagged UFBP1 M4 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1 F196A V198A
Plasmid#204564PurposeExpresses GST-tagged UFBP1 F196A V198A in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-MVP
Plasmid#204541PurposeExpresses GST-tagged MVP in E.coliDepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78
Plasmid#193367PurposeExpression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA
Plasmid#175050PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3e_NAB
Plasmid#156473PurposeBacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tagDepositorInsertNsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-FX
Plasmid#111272Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
ExpressionBacterialMutationinterdomain A (Phe 119 to His 162) substituted by…PromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOG40-HRS (257-276) Hs
Plasmid#83236PurposepGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coliDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only