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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Bacterial

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight cz::caspase-3 (p17) [TU#818]

Plasmid

#16085

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Expression

Mammalian

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pLTR-G

Plasmid

#17532

Depositor

Jakob Reiser

Article

Reiser et al Proc Natl Acad Sci U S A. 1996 Dec 24. 9

Insert

VSV-G glycoprotein (Indiana strain)

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mKelly1

Plasmid

#119782

Purpose

Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-Neo HA-hSki

Plasmid

#10910

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

Ski (SKI Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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FKBP-eGFP-TAZ

Plasmid

#221213

Purpose

part A of TAZ-SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-eGFP-TAZ

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-KO

Plasmid

#20331

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV puro let-7 sponge

Plasmid

#29766

Depositor

Phil Sharp

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

let-7 sponge

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pCEFL3xHAmGli2

Plasmid

#37671

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-2

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

H2B-LSSmKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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p416 25Q GPD

Plasmid

#1177

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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p5T7-LYCipi-ggpps

Plasmid

#122017

Purpose

Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Insert

GGPP synthase

Expression

Bacterial

Mutation

codon optimized, truncated first 98 amino acids, …

Promoter

T7

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p416 103Q GPD

Plasmid

#1180

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
Clathrin-LCa-EYFP

Plasmid

#21741

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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Pmec-18 cz::caspase-3 (p17) [TU#814]

Plasmid

#16083

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal-RNQ1-YFP

Plasmid

#18686

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pSAC5.02

Plasmid

#16077

Depositor

Jochen Buck, Lonny Levin

Article

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Expression

Mammalian

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
Phsp-16 cz::caspase-3 (p17) [TU#823]

Plasmid

#16086

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mClover3

Plasmid

#133453

Purpose

Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mClover3

Tags

GSGSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFSy(1.1)GW

Plasmid

#27232

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pFLAG-CMV4-ZIKV-PrM

Plasmid

#178840

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-PrM

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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[pSK581] pRK5 HA-SLC38A9N

Plasmid

#136141

Purpose

mammalian expression of SLC38A9 N term fragment (1-119)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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