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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,282 results for: 261

Showing: 1321 - 1360 of 4282 results

  7. pKG96

    Plasmid
    #63072
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-A (CENPA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    A12V, C75Y, and L121F mutations compared to GenBa…
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63072-plasmid-map-sequence-id-134723

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  10. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  11. R777-E061 Hs.ERBB2

    Plasmid
    #70345
    Purpose
    Gateway ORF clone of human ERBB2 [NM_004448.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ERBB2 (ERBB2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70345-plasmid-map-sequence-id-125794

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  15. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  20. CSADA

    Plasmid
    #42387
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSADA (CSAD Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. GPX8

    Plasmid
    #38917
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX8 (GPX8 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. p663-UBC-miniTurbo-V5-PHKG2_IDG-K

    Plasmid
    #158186
    Purpose
    Gateway destination clone of PHKG2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PHKG2 (PHKG2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158186-plasmid-map-sequence-id-314662

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  25. pECT1

    Plasmid
    #75446
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75446-plasmid-map-sequence-id-146474

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  36. EPB41L3A-c021

    Plasmid
    #175460
    Purpose
    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175460-plasmid-map-sequence-id-353154

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  38. pHH0103 MAGI1 WW domain #1

    Plasmid
    #104242
    Purpose
    Bacterial expression of WW domain #1 from MAGI1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    MAGI1 WW domain #1 (MAGI1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104242-plasmid-map-sequence-id-204800

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  39. pHH0103 MAGI1 WW domain #2

    Plasmid
    #104243
    Purpose
    Bacterial expression of WW domain #2 from MAGI1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    MAGI1 WW domain #2 (MAGI1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104243-plasmid-map-sequence-id-204294

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  40. pECT1B

    Plasmid
    #75447
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75447-plasmid-map-sequence-id-146475

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Showing: 1321 - 1360 of 4282 results