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pBCiG2

Plasmid

#149470

Purpose

Broad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSSec7-mGFPx3

Plasmid

#25449

Depositor

Benjamin Glick

Article

Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Insert

Sec7 (SEC7 Budding Yeast)

Tags

mGFPx3

Expression

Yeast

Mutation

GFP contains A206K for monomerization.

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pFLxR1

Plasmid

#149496

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKNR5

Plasmid

#149466

Purpose

Broad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pKCyR5

Plasmid

#149463

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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HS-H3-GFP

Plasmid

#8698

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3 (His3:CG31613 Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pILxR5

Plasmid

#149497

Purpose

Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFLtR5

Plasmid

#167505

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtetO-1

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKT0090

Plasmid

#8714

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yEVenus

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pBLxR5

Plasmid

#167504

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pL2/GZ

Plasmid

#1282

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GRE-lacZ

Tags

glucocorticoid response elemen-lacZ

Expression

Yeast

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCD315

Plasmid

#113321

Purpose

pBAD-W108 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pFVTd5

Plasmid

#167520

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-tdKatushka2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFVR5

Plasmid

#149489

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLlacO1-deGFP-T500

Plasmid

#45394

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

PL-lacO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pFCyR5

Plasmid

#149480

Purpose

Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p231 pCMVe-betaAc-STOP-luc

Plasmid

#12463

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc-STOP

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pRB39

Plasmid

#72870

Purpose

template for N-terminal eGFP tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB40

Plasmid

#72865

Purpose

template for N-terminal eGFP tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLUC

Plasmid

#1280

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

CUP1-lacZ fusion (CUP1-1 Budding Yeast)

Tags

CUP1 promoter

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pFPcR5

Plasmid

#149484

Purpose

Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-FKBP(M)x4

Plasmid

#85004

Purpose

Expresses mEGFP-FKBP(M)x4

Depositor

Benjamin Glick

Article

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

EGFP-FKBP(M)x4

Expression

Yeast

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG49

Plasmid

#164707

Purpose

yEVenus under the control of GAL1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GAL1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFCiK5

Plasmid

#167514

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PCx35-pcDNA

Plasmid

#42920

Depositor

John O'Brien

Article

O'Brien et al J Neurosci. 2004 Jun 16;24(24):5632-42.

Insert

Connexin 35

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pJ2077.2

Plasmid

#140666

Purpose

Controller plasmid for TS circuit and cL and cR controls

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Controller plasmid for TS circuit and cL and cR controls

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK41

Plasmid

#74886

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
E2-Crimson-FKBP(M)

Plasmid

#85002

Purpose

Expresses E2-Crimson-FKBP(M)

Depositor

Benjamin Glick

Article

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(M)

Expression

Yeast

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST-p15A-PLlacO1-lacI-T500

Plasmid

#45393

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

lac repressor

Expression

Bacterial

Promoter

PL-lacO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT055g

Plasmid

#96864

Purpose

sgRNA-HEK3

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK3

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB34

Plasmid

#72862

Purpose

template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
C-Kras

Plasmid

#27807

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Kras

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-Control-miR-20-Sensor

Plasmid

#21168

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Type

Empty backbone

Expression

Mammalian

Available Since

June 10, 2009

Availability

Academic Institutions and Nonprofits only
pKCyGe2

Plasmid

#167515

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymRAM-GmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBVR5

Plasmid

#167511

Purpose

Broad-Host range expression vector, pBBR origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG45

Plasmid

#164703

Purpose

yEVenus under the control of CUP1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

CUP1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1809_pmax-RBM38-FRB

Plasmid

#118642

Purpose

Transient transfection; Expresses RBM38-FRB; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-FRB

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426Gal-GFP

Plasmid

#38310

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
HS-H3.3A-GFP

Plasmid

#8696

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A (His3.3A Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1810_pmax-FRB-RBM38

Plasmid

#118643

Purpose

Transient transfection; Expresses FRB-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FRB-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOX + kqt1

Plasmid

#16136

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-1 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
SI1-Unc5b

Plasmid

#28214

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Unc5b mouse monoclonal antibody (unc5b Zebrafish)

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAV12

Plasmid

#67713

Purpose

Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Kieft et al RNA. 2004 Jun;10(6):988-95.

Insert

Transcription cassette for 3'-tagged RNAs

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF297

Plasmid

#74774

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF292

Plasmid

#72856

Purpose

template base vector for N-terminal tagging, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for Nterm-tag templates

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SI2-Unc5b

Plasmid

#28215

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Unc5b mouse monoclonal antibody (unc5b Zebrafish)

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
bfloGFPc1a

Plasmid

#60750

Purpose

Bacterial expression of Amphioxus GFPc1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPc1

Tags

8xHIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only

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