We narrowed to 2,171 results for: Endog

Showing: 661 - 680 of 2171 results

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pX330-PCNT

Plasmid

#227284

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of PCNT (PCNT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Tags

mEGFP

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJL257

Plasmid

#243696

Purpose

A piggyBac vector containing the NDUFB9 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of NDUFB9 (NDUFB9 Human)

Expression

Mammalian

Promoter

endogenous promoter

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-Siwi

Plasmid

#50560

Purpose

Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

Siwi (Aubergine )

Tags

6xHIS and FLAG

Expression

Insect

Promoter

OpIE2

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-CRBNmidi-miniTurbo-3xFLAG

Plasmid

#248675

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo CRBNmidi for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

CRBNmidi

Tags

NLS and miniTurbo-3x FLAG

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-His6-ODC-Ctag

Plasmid

#163614

Purpose

Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

ODC (ODC1 Human)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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(401) pAAV CMV-ZsGreen U6-gRNA

Plasmid

#163021

Purpose

Fluorescent reporter for Sa gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Zs Green

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F2

Plasmid

#184164

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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