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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 661 - 680 of 11938 results

  2. pCS-H2B-cerulean

    Plasmid
    #53748
    Purpose
    Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.
    Depositor
    Sean Megason
    Publication
    Megason et al Methods Mol Biol. 2009;546:317-32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone H2B (H2BC21 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53748-plasmid-map-sequence-id-83988

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  4. pcDNA3-Lyn-ICUE3

    Plasmid
    #61623
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring cAMP
    Depositor
    Jin Zhang
    Publication
    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-ICUE3 (RAPGEF3 Human)
    Tags
    ECFP, N-terminal targeting sequence from Lyn kina…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61623-plasmid-map-sequence-id-100424

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  5. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid
    #58475
    Purpose
    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58475-plasmid-map-sequence-id-90385

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  7. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  9. PUF Assembly Kit

    Plasmid kit
    #1000000051
    Purpose
    Assembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.
    Depositor
    Huimin Zhao
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Bacterial Expression, Mammalian Expression
    Editing type
    PUF
    Cloning type
    Golden Gate
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108867-plasmid-map-sequence-id-209739

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  11. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV5)

    Viral prep
    #198511-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet (Cre-dependent)
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 661 - 680 of 11938 results