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pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pEPQD1CB0107

Plasmid

#177040

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pp32ΔCT

Plasmid

#67241

Purpose

pp32(1-149) cloned into pProEX HTb

Depositor

Cynthia Wolberger

Article

Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

Insert

pp32 (ANP32A Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 1-149

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChromeQ-mCherry

Plasmid

#123125

Purpose

AAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

GFAP104

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(WT)-EGFP

Plasmid

#79407

Purpose

Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
mCerulean3-Vimentin-7

Plasmid

#55452

Purpose

Localization: Intermediate Filaments, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vimentin (VIM Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pUCHR-CD5Flag2-P2A-smAID-mClover

Plasmid

#110657

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5Flag2-P2A-AID-mClover

Use

Lentiviral

Promoter

CMV

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron(Neo)-G(FLLFLNL)

Plasmid

#40021

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (ATG)
GFP
beta-globin intron
Neo

Mutation

deleted nucleotides 1-274 and insertion of FRT, …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas delCAAX-IP

Plasmid

#13834

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas delCAAX (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Deletion 4 amino acids of c-terminal end (CAAX mo…

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pUC19 PKC epsilon

Plasmid

#8442

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas C220S/C222S-IP

Plasmid

#13829

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas C220S/C222S (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C220 and C222 changed to serine residues

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pJ207 Delta-cpc-KanR

Plasmid

#51468

Purpose

Deletes the cpc operon in Synechocystis and confers kanamycin resistance

Depositor

Anastasios Melis

Article

Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

Insert

Replacing the cpc operon with kanamycin resistance

Use

Synthetic Biology

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170817

Purpose

Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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YF-Cav1s

Plasmid

#171462

Purpose

Expresses YF-Cav1s, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP-FKBP12-Caveolin1(61-178 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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mActZ-FY

Plasmid

#171463

Purpose

Expresses mActZ-FY, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas SSVA-IP

Plasmid

#13827

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas SSVA (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C224 changed to serine

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

Plasmid

#82469

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

changed Lysine 294 to Alanine

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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