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pET28a(+)_beta2M (MHC Class I light)

Plasmid

#187357

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

beta2M (MHC Class I light)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D NES-jRCaMP1a

Plasmid

#171120

Purpose

Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jRCaMP1a

Use

AAV

Tags

His Tag, T7 Tag, XpressTag

Expression

Mammalian

Promoter

gfaABC1D

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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strep-IRF8

Plasmid

#102872

Purpose

Express strep-tagged mouse IRF8 protein in E. coli

Depositor

Gary Stormo

Article

Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

Insert

IRF8 (Irf8 Mouse)

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK4

Plasmid

#122675

Purpose

Expresses mouse PCSK4 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_ancCas9d_Plasmid

Plasmid

#239444

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Article

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

ancCas9d

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Traptavidin-E6

Plasmid

#59549

Purpose

pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

Depositor

Mark Howarth

Article

Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

Insert

Core Traptavidin-E6

Tags

E6 tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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PET-28a-YTH domain of YTHDF2

Plasmid

#228776

Purpose

Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

Depositor

Huiqing Zhou

Article

Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

Insert

YTH domain of YTHDF2 (YTHDF2 Human)

Tags

6*His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Article

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C)

Plasmid

#79045

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C)

Expression

Bacterial

Mutation

A14C and E45C mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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TAL3216

Plasmid

#42679

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-left (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only