We narrowed to 7,696 results for: CCH

1. pST1374-dCas9-GCN4

   Plasmid

   #113026

   Purpose

   Expresses dCas9-GCN4 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)
   

   Expression

   Mammalian

   Mutation

   D10A, H840A, N863A

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184738

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN-G1Gh

   Plasmid

   #44507

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSc-His6-Sem1Sc

   Plasmid

   #83235

   Purpose

   pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Sem 1 (SEM1 Budding Yeast)
   

   Tags

   His6

   Expression

   Yeast

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyG3_hph

   Plasmid

   #184919

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN HA-ATG13

   Plasmid

   #186223

   Purpose

   For expression of ATG13 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS405 PEX14-VAC8-RFP

   Plasmid

   #166844

   Purpose

   Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, PEX14 Budding Yeast)
   

   Tags

   PEX14 transmembrane domain and RFP

   Expression

   Yeast

   Mutation

   PEX14 transmembrane domain

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS405 SEC66-VAC8-RFP

    Plasmid

    #166843

    Purpose

    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Tags

    RFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184739

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. nuc-yHS1-M7A

    Plasmid

    #159167

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171616

    Purpose

    Soma-targeted Cal-Light with Kv2.1

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MED9

    Plasmid

    #15407

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Expression

    Bacterial and Yeast

    Promoter

    pADH1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN-T2dGZmxh

    Plasmid

    #44552

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120393

    Purpose

    pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Diras2 shRNA (Diras2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG2_zeo

    Plasmid

    #184913

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge