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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,388 results for: ACE;

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Showing: 6961 - 6980 of 21388 results

  2. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  3. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  8. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

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  10. pAP05

    Plasmid
    #186261
    Purpose
    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    Expression
    Bacterial
    Promoter
    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186261-plasmid-map-sequence-id-369315

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  19. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227327-plasmid-map-sequence-id-452117

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Showing: 6961 - 6980 of 21388 results