We narrowed to 7,696 results for: Pol

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pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Publication

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Publication

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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POLArIS_NbVim-cp10GFP

Plasmid

#171020

Purpose

Expresses NbVim-cp10GFP#2 in mammalian cells

Depositor

Sumio Terada

Publication

Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

Insert

anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)

Tags

cp10GFP

Expression

Bacterial and Mammalian

Promoter

CMV promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_1

Plasmid

#160762

Purpose

Suppress POLE1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA4/TO-ORF24-3xFLAG

Plasmid

#138423

Purpose

Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Publication

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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AP1muA-TurboID-polyA-G418 HR

Plasmid

#227734

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Publication

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Turbo-ID, V5

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL3.7-EF-EYFP-YAP1_S94A-PolyA

Plasmid

#112286

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Serine 94 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-POLR2G-GFP

Plasmid

#86872

Purpose

To express POLR2G (RPB7) tagged with VENUS at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

POLR2G (POLR2G Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Publication

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pFIV-H1-Puro-hPolß

Plasmid

#18663

Depositor

Robert Sobol

Publication

Trivedi et al Molecular Pharmacology 2008;74(2):505-16

Insert

shRNA specific to human DNA polymerase beta (POLB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

none

Available since

Oct. 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

Plasmid

#220224

Purpose

AAV-STARR-seq validation control vector with Venus

Depositor

Simon Rumpel

Publication

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDmPolyUbiq

Plasmid

#165786

Purpose

Drosophila Polyubiquitin promoter, strongest promoter in the library

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

PolyUbiq

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.17_GagZip-MCP-dpol-P2A-eGFP

Plasmid

#205532

Purpose

Express HIV GagZip-MCP-dpol-P2A-eGFP

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP-dpol

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, pol fr…

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Cyto-mTurq2-Apollo-NADP+

Plasmid

#193301

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

Depositor

Jonathan Rocheleau

Publication

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G6PD (G6PD Human)

Tags

mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Publication

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Hs PEG10-Dendra2 gag-pol isoform3

Plasmid

#260100

Purpose

Expression of Human PEG10-dendra2 isoform3 and CFP using IRES

Depositor

Alexandra Whiteley

Publication

Black et al Elife. 2023 Mar 23;12:e79452. doi: 10.75

Insert

PEG10 (PEG10 Human)

Tags

dendra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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Hs PEG10-dendra2 gag-pol (D370A)

Plasmid

#260096

Purpose

Expression of Human proteolytically inactive PEG10-dendra2 and CFP using IRES

Depositor

Alexandra Whiteley

Publication

Roberts et al J Cell Sci. 2025 Dec 15;138(24):jcs26410

Insert

PEG10 (D370A) (PEG10 Human)

Tags

dendra2

Expression

Mammalian

Mutation

PEG10 A1108C

Promoter

CMV

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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