161,398 results

1. pAAV-Ucp1-Cre-miR122

   Plasmid

   #228408

   Purpose

   Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

   Depositor

   Joerg Heeren

   Article

   Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

   Inserts

   cre recombinase with SV40 NLS
   Cre recombinase with SV40 NLS
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   rat Ucp1 enhancer-promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS1995

   Plasmid

   #49125

   Purpose

   AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple94-iCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GPR88

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Use

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Hph

   Plasmid

   #188741

   Purpose

   pUC19 plasmid (MCS intact) with Hygromycin selection marker

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   Hygromycin
   

   Use

   Filamentous fungal expression

   Tags

   Expression

   Mutation

   Promoter

   gpdA

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CMV-mC-G-LC3B-P

   Plasmid

   #124974

   Purpose

   evaluate autophagy

   Depositor

   Kazuhiro Oka

   Article

   Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

   Insert

   mCherry-GFP-LC3B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. phOCT4-Luc

   Plasmid

   #17221

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   Upstream region of human OCT4 (POU5F1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK031.TRE-Cre (Supernova)

   Plasmid

   #69136

   Purpose

   For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

   Depositor

   Takuji Iwasato

   Article

   Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

   Insert

   NLS-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-CD68 promoter/enhancer

    Plasmid

    #34837

    Depositor

    Peter Murray

    Article

    Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

    Insert

    CD68 promoter/enhancer -680/+140 (CD68 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC3_C_VPS35L

    Plasmid

    #209698

    Purpose

    Expresses MAC3-tagged VPS35L in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

    Insert

    VPS35L (VPS35L Human)
    

    Use

    Tags

    MAC3-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-15b-UbcH5b/2419

    Plasmid

    #11475

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5b (UBE2D2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

14. yo-msGFP2

    Plasmid

    #160471

    Purpose

    Encodes a yeast codon optimized version of msGFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    yo-msGFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-FHH-Empty Vector-IRES-Puro

    Plasmid

    #120568

    Purpose

    For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag-HA-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGH125_dCas9-Blast

    Plasmid

    #85417

    Purpose

    lentiviral expression vector for dCas9 with Blasticidin selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    dCas9 and Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AKT-translocation-mStrawberry reporter

    Plasmid

    #158685

    Purpose

    This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-6XHis-human SUMO 2

    Plasmid

    #133771

    Purpose

    express 6Xhis- SUMO 2 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 2 (SUMO2 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM2s2AsG

    Plasmid

    #137924

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Flpe:GFP

    Plasmid

    #13788

    Purpose

    Mammalian expression of enhanced form of Flp recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe-GFP fusion protein
    

    Use

    Flp/frt

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-eNme2-T.1-ABE8e

    Plasmid

    #185668

    Purpose

    Expresses eNme2-T.1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. TFORF1938

    Plasmid

    #141892

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MEIS1 (MEIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. PH-PLCd1-mNGx1

    Plasmid

    #202751

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170):mNG2 (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. His-GFP-Syntaxin1A

    Plasmid

    #170871

    Purpose

    Mammalian expression of His-GFP-Syntaxin1A.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-Ef1a-mCherry

    Plasmid

    #114470

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-S5E2-dTom-nlsdTom

    Plasmid

    #135630

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIRE4-Bpa

    Plasmid

    #155342

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    humanized EBpaRS/BstYam
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/U6

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMSCV-IRES-GFP-human-Caspase-4

    Plasmid

    #183356

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge