165,192 results

1. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

   Plasmid

   #233080

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1i

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP1692 - pAAV-AiE2367m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #220661

   Purpose

   AiE2367m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

   Plasmid

   #222853

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G, H435A, I253A, H310A

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

   Plasmid

   #222855

   Purpose

   Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G, H435A, I253A, H310A

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgYAP-TAZ-2

   Plasmid

   #229452

   Purpose

   knockout of YAP1 and WWTR1 (TAZ)

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgYAP-TAZ-2 (YAP1, WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Use

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLYS6-METTL17-CtoS-FLAG

   Plasmid

   #220876

   Purpose

   Expressing METTL17-FLAG_Cys to Ser mutants

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Cys to Ser

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. kiCAP-AAV-BI-hTFR1-3

   Plasmid

   #218798

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1-3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-REPORT (PGK) 12xHRE-NanoLuc

    Plasmid

    #208575

    Purpose

    hypoxic response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    12xHRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Blast-mNeon-ACTB

    Plasmid

    #207751

    Purpose

    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2370

    Plasmid

    #142056

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MBD4 (MBD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSII(KSplus)-HsBGG-WT_E

    Plasmid

    #146187

    Purpose

    Bacterial Expression of HsBGG-WT

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsBGG-WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-MUC2 D1 with cleavable His tag

    Plasmid

    #181813

    Purpose

    Expresses human MUC2 D1 with cleavable His tag, residues 21-389

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc2 D1 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL1P1_AtU626Ter26

    Plasmid

    #191769

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. RCP83

    Plasmid

    #163466

    Purpose

    Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

    Depositor

    Frank Albert

    Article

    Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

    Inserts

    Promoter fragment (Sharon2012_1922)
    His3 minimal promoter
    yeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. p7SKU6SBpur

    Plasmid

    #160329

    Purpose

    dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Type

    Empty backbone

    Use

    Tags

    cfSBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB1A3_GFP_dT25

    Plasmid

    #89664

    Purpose

    expression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSB1A3_GFP_dT19

    Plasmid

    #89661

    Purpose

    expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT19
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Gg 3kb Green opsin GFP

    Plasmid

    #72919

    Purpose

    green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    chicken green opsin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLH-sgRNA1-2XMS2

    Plasmid

    #75389

    Purpose

    sgRNA1-2XMS2

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMV306hsp

    Plasmid

    #26155

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CamKII-LGI1-pHmScarlet

    Plasmid

    #185538

    Purpose

    Expresses LGI1-pHmScarlet under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHmScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBR BsmAIM Kan + p15A BsaIR Amp

    Plasmid

    #206208

    Purpose

    Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM and BsaIR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge